Ghosh P, Singh U N
Department of Biophysics, University of Delhi, India.
Cell Biol Int. 1997 Sep;21(9):551-7. doi: 10.1006/cbir.1997.0193.
Glial cells in the brain are known to provide structural and functional supports to neurons. To sustain such a supportive role, they have developed cell-to-cell communicating gap junctional channels. The authors studied the effect of dbcAMP on gap junctional channels mediated communication in C6 cells, a rat glioma cell line. Quantitative assessment of coupled cells under microscope after microinjection of a fluorescent dye was taken as a measure of junctional permeability. An enhanced coupling between cells was observed following dbcAMP treatment and this elevated coupling was found to be dependent on the duration of exposure of cells to dbcAMP. The studies have focused on a subtle shift in the spatial organization of the functional channels to the processes of dbcAMP induced differentiated cells from the cell cytoplasms and membranes of dbcAMP untreated cells. Immunofluorescence study with affinity purified antibody against gap junction further confirmed the spatial distribution of gap junctional protein(s) in the processes and also showed an increase in the density of the protein at the intercellular spaces in dbcAMP induced differentiated C6 glioma cells.
已知大脑中的胶质细胞为神经元提供结构和功能支持。为维持这种支持作用，它们形成了细胞间通讯的间隙连接通道。作者研究了二丁酰环磷腺苷（dbcAMP）对大鼠胶质瘤细胞系C6细胞中介导间隙连接通道通讯的影响。显微注射荧光染料后，在显微镜下对耦联细胞进行定量评估，以此作为连接通透性的指标。dbcAMP处理后观察到细胞间耦联增强，且这种增强的耦联取决于细胞暴露于dbcAMP的持续时间。这些研究聚焦于功能性通道空间组织的细微变化，即从dbcAMP未处理细胞的细胞质和细胞膜到dbcAMP诱导分化细胞的过程。用针对间隙连接的亲和纯化抗体进行免疫荧光研究，进一步证实了间隙连接蛋白在这些过程中的空间分布，并且还显示在dbcAMP诱导分化的C6胶质瘤细胞的细胞间隙中蛋白质密度增加。
