通过酶联免疫吸附测定法（ELISA）和蛋白质免疫印迹法（western blotting）对人、小鼠和兔抗麦醇溶蛋白抗体进行表征。
Characterization of human, mouse and rabbit anti-gliadin antibodies by ELISA and western blotting.
作者信息
Vancíková Z, Kocna P, Tucková L, Fric P, Dvorák M, Stoyanov S, Tlaskalová-Hogenová H
机构信息
Department of Clinical Immunology, Faculty Hospital Bulovka, Prague, Czech Republic.
出版信息
Folia Microbiol (Praha). 1995;40(6):659-64. doi: 10.1007/BF02818526.
Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, alpha-, beta- and omega-gliadins was found in the patients' sera than in controls.
采用酶联免疫吸附测定（ELISA）和免疫印迹技术分析了单克隆、超免疫兔和人血清抗麦醇溶蛋白抗体。在蛋白质印迹法中，单克隆抗体和人抗体之间的反应性差异是定量的而非定性的。兔抗血清的反应性因用于免疫的蛋白质而异。用麦醇溶蛋白的胰蛋白酶 - 胃蛋白酶消化产物免疫的兔子与患者的反应相似。在ELISA中，患者血清对粗麦醇溶蛋白、A - 麦醇溶蛋白、糖基化麦醇溶蛋白、α - 麦醇溶蛋白、β - 麦醇溶蛋白和ω - 麦醇溶蛋白的反应性显著高于对照组。
Zotero 插件