与麦醇溶蛋白反应的单克隆抗体作为评估导致乳糜泻病情加重的抗原结构的工具。
Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease.
作者信息
Stoyanov S, Tlaskalová-Hogenová H, Kocna P, Kristofová H, Fric P, Hekkens W T
机构信息
Institute of Molecular Genetics, Czechoslovak Academy of Science, Prague.
出版信息
Immunol Lett. 1988 Apr;17(4):335-8. doi: 10.1016/0165-2478(88)90007-7.
PMID:3372012
Abstract
Monoclonal antibodies reactive with gliadin were prepared by fusion of spleen cells isolated from gliadin-immunized BALB/c mice with myeloma cells. The reactivity of mAbs with different preparations of gliadin and their enzymatic digest were measured using ELISA method. The mAb produced by GL 1 clone was shown to react preferentially with alpha-gliadin and its enzymatic digest.
摘要
通过将从麦醇溶蛋白免疫的BALB/c小鼠分离的脾细胞与骨髓瘤细胞融合，制备了与麦醇溶蛋白反应的单克隆抗体。使用酶联免疫吸附测定（ELISA）方法测量单克隆抗体与不同麦醇溶蛋白制剂及其酶消化产物的反应性。结果显示，由GL 1克隆产生的单克隆抗体优先与α-麦醇溶蛋白及其酶消化产物发生反应。
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