Pavlova I S, Liubavina I A, Lukin Iu V
Bioorg Khim. 1996 Apr;22(4):269-72.
Simple methods to determine 2,4-dichlorophenoxyacetic acid (2,4-D) using biotinylated monoclonal antibodies in combination with a highly active streptavidin-peroxidase conjugate were developed on the basis of the immunofiltration dot assay and competitive enzyme immunoassay (EIA). The detection limit of 2,4-D acid for immunofiltration and EIA was 2-3 ng/ml, the total assay duration being 5 min and 1.5 h, respectively.
基于免疫过滤斑点分析和竞争性酶免疫分析（EIA），开发了使用生物素化单克隆抗体与高活性链霉亲和素-过氧化物酶偶联物相结合来测定2,4-二氯苯氧乙酸（2,4-D）的简单方法。免疫过滤和EIA对2,4-D酸的检测限为2-3 ng/ml，总分析时间分别为5分钟和1.5小时。
