Gordienko A I, Krivoshein Iu S, Malyĭ K D, Klimovich V B, Gervazieva V B
Mikrobiol Z. 1996 Mar-Apr;58(2):75-80.
A "sandwich"-variant of the solid phase immunoenzyme method of quantitative determination of human IgE in biological liquids has been developed on the basis of two monoclonal antibodies to various epitopes of Fc-fragment of E-chain of human IgE and biotin-streptavidin system of the signal amplification. The total analysis time is 3 h: the range of determined concentrations of IgE is 1-200 kE/l; sensitivity-1 kE/l; the type of calibrating curve is linear. To make an analysis twice 10 microliters of the tested sample are required. Reproducibility of the suggested method is not below 5%.
基于针对人IgE重链Fc片段不同表位的两种单克隆抗体以及信号放大的生物素-链霉亲和素系统，开发了一种用于定量测定生物液体中人IgE的固相免疫酶法的“夹心”变体。总分析时间为3小时；IgE测定浓度范围为1 - 200 kE/l；灵敏度为1 kE/l；校准曲线类型为线性。进行一次分析需要10微升待测样品。所建议方法的重现性不低于5%。
