Man'ko V M, Guseva O A, Miroshnichenko I V, Iarilin A A
Biull Eksp Biol Med. 1992 Feb;113(2):170-2.
The influence of B-lymphocytes from various sources on splenic colony formation was studied in the syngeneic system. B-lymphocytes were obtained by panning with IgG-fraction of rabbit anti-mouse Ig, absorbed on Petri dishes. In addition, adherent cells, Thy-1+ and SC-1+ were eliminated from the fraction of Ig(+)-cells. SC-1- and SC-1+ fractions, containing, respectively, stem cells and T-lymphocyte precursors, were obtained by panning with IgG-fraction of rabbit anti-SC-1 serum. SC-1- cells transferred to irradiated syngeneic mice did not induce colony formation in the spleen. Introduction of SC-1- and SC-1+ cells induced formation of colonies. A similar helper effect occurred when SC-1(-)-cells were introduced with bone marrow or lymph node B-cells, but not with splenic B-cells. Splenic, but not bone marrow and lymph node B-cells inhibited colony formation by combination of SC-1- and SC-1+ cells. All effects of Ig+ cells were abolished by treatment of cells with rabbit anti-MBLA serum. Thus, B-cells of various origin can either enhance or inhibit colony formation. The enhancing of inhibitory effect after B (MBLA+)-cells elimination from suspension of bone marrow and lymph node (but not spleen) Ig(+)-cells resulted from the activity of B-contrasuppressors.
在同基因系统中研究了来自不同来源的B淋巴细胞对脾集落形成的影响。通过用吸附在培养皿上的兔抗小鼠Ig的IgG组分进行淘选获得B淋巴细胞。此外，从Ig(+)细胞组分中去除了贴壁细胞、Thy-1+和SC-1+细胞。通过用兔抗SC-1血清的IgG组分进行淘选，分别获得了含有干细胞和T淋巴细胞前体的SC-1-和SC-1+组分。将SC-1-细胞转移到经照射的同基因小鼠中不会诱导脾脏中的集落形成。引入SC-1-和SC-1+细胞可诱导集落形成。当将SC-1(-)细胞与骨髓或淋巴结B细胞而非脾B细胞一起引入时，会出现类似的辅助效应。脾B细胞而非骨髓和淋巴结B细胞通过SC-1-和SC-1+细胞的组合抑制集落形成。用兔抗MBLA血清处理细胞可消除Ig+细胞的所有效应。因此，不同来源的B细胞可以增强或抑制集落形成。从骨髓和淋巴结（而非脾脏）Ig(+)细胞悬液中去除B(MBLA+)细胞后，抑制作用增强是由B反抑制因子的活性所致。
