Palomar Lever A, Furuya Meguro M E, Gómez González A, Martínez Guerra M L, Gertrudiz Salvador N, Oppenheimer L, Sandoval Zárate J
Departamento de Cardioneumología, Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Arch Inst Cardiol Mex. 1996 Jan-Feb;66(1):10-22.
The aim of this paper is to introduce the spectrophotometric method to the study of pulmonary edema in isolated ex-vivo canine pulmonary lobe preparation. This spectrophotometric method is based on the on-line measure of light transmission in a column of blood, that is proportional to hematocrit. A second light is used to follow Evans blue dyed proteins. With this method we were able to measure the amount of edema in 10 isolated canine lobes. Both the filtration and reflection coefficient of the membrane as well as the characteristics of the filtrate could be calculated. The filtration coefficient was 0.6 +/- 0.4 ml/min (1.3 +/- 0.9 ml/min/100 g pulmonary, tissue) at maximum capillary pressure and the reflection coefficient was 0.53 +/- 0.07. With the spectrophotometric method we have the capability to study different aspects of lung edema formation. This method has the advantage of being exact and independent from pressure and volume induced vascular changes. It also allows the measurement of solute transport.
本文旨在将分光光度法引入离体犬肺叶制备中肺水肿的研究。这种分光光度法基于对一列血液中光透射率的在线测量，该光透射率与血细胞比容成正比。使用另一束光来追踪伊文思蓝染色的蛋白质。通过这种方法，我们能够测量10个离体犬肺叶中的水肿量。可以计算膜的滤过系数和反射系数以及滤液的特性。在最大毛细血管压力下，滤过系数为0.6±0.4 ml/min（1.3±0.9 ml/min/100 g肺组织），反射系数为0.53±0.07。使用分光光度法，我们有能力研究肺水肿形成的不同方面。该方法的优点是精确，且不受压力和容量引起的血管变化的影响。它还允许测量溶质转运。
