Knoblett J N, Youssef N N
Department of Biology, Utah State University, Logan, UT 84322-5305, USA.
J Econ Entomol. 1996 Aug;89(4):841-7. doi: 10.1093/jee/89.4.841.
We developed several monoclonal antibodies that recognized germinated and nongerminated spores and other developmental stages of Nosema locustae, a pathogen of grasshoppers, by using spore homogenate as antigen. The hybridomas were produced with standard techniques. Indirect fluorescence assay demonstrated recognition by several antibodies to the spore and extruded polar filament. Monoclonal antibody D10-9-11 recognized the spore wall in smears and frozen sections of infected grasshopper fat body tissue, whereas monoclonal antibody D55-12 also labeled the extruded polar filament. Immunoelectron microscopy showed that monoclonal antibody D10-9-11 recognized the spore and several developmental stages. Enzyme-linked immunosorbent assay showed that monoclonal antibody D10-9-11 did not cross-react with immunogen prepared from other grasshopper pathogens, including Vairimorpha sp., Malameba locustae, and entomopox virus from migratory grasshoppers, Melanoplus sanguinipes (F.).
我们以孢子匀浆为抗原，制备了几种单克隆抗体，这些抗体可识别蝗虫微孢子虫（一种蝗虫病原体）的已萌发和未萌发的孢子以及其他发育阶段。采用标准技术制备杂交瘤。间接荧光试验表明，几种抗体可识别孢子和伸出的极丝。单克隆抗体D10-9-11在感染蝗虫脂肪体组织的涂片和冰冻切片中可识别孢子壁，而单克隆抗体D55-12也可标记伸出的极丝。免疫电子显微镜显示，单克隆抗体D10-9-11可识别孢子和几个发育阶段。酶联免疫吸附测定表明，单克隆抗体D10-9-11与从其他蝗虫病原体制备的免疫原无交叉反应，这些病原体包括变形孢虫属、蝗虫马拉霉以及来自迁徙蝗虫红翅蝗（Melanoplus sanguinipes (F.)）的昆虫痘病毒。
