伏马菌素B1对膜中氧气运输的影响。

Effects of fumonisin B1 on oxygen transport in membranes.

作者信息

Yin J J, Smith M J, Eppley R M, Page S W, Sphon J A

机构信息

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204, USA.

出版信息

Biochem Biophys Res Commun. 1996 Aug 5;225(1):250-5. doi: 10.1006/bbrc.1996.1162.

DOI:10.1006/bbrc.1996.1162

PMID:8769126

Abstract

Electron spin resonance (ESR) spin-label oximetry has been used to study the effects of fumonisin B1 (FB1), a sphingoid-like mycotoxin, on oxygen transport in phosphatidylcholine (PC) bilayers. Moreover, the use of spin labels attached to different carbons of fatty acids makes it possible to do structural and oximetric determinations with the same test sample. Specifically, the incorporation of 10 mol% FB1 increased the oxygen transport properties of both saturated and unsaturated membranes at 37 degrees C by ca. 30% and decreased the ordering of the hydrocarbon chains near the surface of the membranes; concomitantly, oxygen transport near the center of bilayers was diminished slightly, and the relative oxygen diffusion-concentration product profile curves were markedly flattened.

摘要

电子自旋共振（ESR）自旋标记血氧测定法已被用于研究伏马菌素B1（FB1，一种类鞘脂霉菌毒素）对磷脂酰胆碱（PC）双层膜中氧转运的影响。此外，使用连接到脂肪酸不同碳原子上的自旋标记，使得对同一测试样品进行结构和血氧测定成为可能。具体而言，掺入10摩尔%的FB1可使饱和和不饱和膜在37摄氏度时的氧转运特性提高约30%，并降低膜表面附近烃链的有序性；与此同时，双层膜中心附近的氧转运略有减少，相对氧扩散-浓度乘积剖面曲线明显变平。