Stonerook M J, Tefend K S, Sharma H M, Peck O C, Wood J D
Department of Physiology, College of Medicine, Ohio State University, Columbus 43210, USA.
Dig Dis Sci. 1996 Aug;41(8):1618-24. doi: 10.1007/BF02087910.
This study measured fecal levels of short-chain fatty acids (SCFAs) relative to the severity of colitis in the cotton-top tamarin model of colitis and colon cancer. Severity of colitis was classified as mild, moderate, or severe by subjective scoring of colonic mucosal biopsies and quantification of inflammatory cell infiltrates in the lamina propria. SCFAs were determined by gas chromatography of SCFAs extracted from fresh feces. Tamarins with moderate or severe colitis had significantly reduced levels of fecal SCFAs. The percent dry matter of feces declined significantly in moderate and severe colitis, while total dry matter output per day increased, indicating that moderate and severe colitis in tamarins was associated with diarrhea and increased fecal water loss. In conclusion, this study found colitis in the tamarin model was associated with decreased fecal SCFA levels and progressive inflammation in a pattern similar to human colitis.
本研究在棉顶狨猴的结肠炎和结肠癌模型中，测量了与结肠炎严重程度相关的粪便短链脂肪酸（SCFA）水平。通过对结肠黏膜活检进行主观评分以及对固有层炎症细胞浸润进行定量分析，将结肠炎的严重程度分为轻度、中度或重度。通过对从新鲜粪便中提取的SCFA进行气相色谱分析来测定SCFA。患有中度或重度结肠炎的狨猴粪便SCFA水平显著降低。中度和重度结肠炎时粪便干物质百分比显著下降，而每日总干物质输出量增加，这表明狨猴的中度和重度结肠炎与腹泻及粪便水分流失增加有关。总之，本研究发现狨猴模型中的结肠炎与粪便SCFA水平降低以及类似于人类结肠炎的进行性炎症有关。
