Rubins J B, Charboneau D, Prigge W, Mellencamp M A
Department of Medicine, VA Medical Center, Minneapolis, Minnesota, USA.
J Infect Dis. 1996 Sep;174(3):507-12. doi: 10.1093/infdis/174.3.507.
Because chronic ethanol ingestion decreases pulmonary clearance of Streptococcus pneumoniae in rats, and extracellular antipneumococcal factors in rat surfactant are important in the early clearance of pneumococci from the rat alveolus, the effects of ethanol ingestion on surfactant bactericidal activity were investigated. Normal surfactant from chow-fed rats showed potent anti-pneumococcal activity, even against bacteria growing in nutrient-rich media under favorable conditions. In contrast, surfactant from ethanol-fed rats and from calorie-restricted control-fed rats had significantly reduced antipneumococcal activity compared with surfactant from chow-fed rats. The reductions in surfactant bactericidal activity produced by ethanol ingestion or caloric restriction did not appear to be mediated through changes in either the total amount or the distribution of fatty acids, the antipneumococcal factors in normal surfactant. Rather, ethanol ingestion, and to a lesser extent caloric restriction, produced a surfactant inhibitor of free fatty acids that was partially characterized as a hydrophobic protein.
由于长期摄入乙醇会降低大鼠肺部对肺炎链球菌的清除能力，且大鼠表面活性剂中的细胞外抗肺炎球菌因子在肺炎球菌从大鼠肺泡的早期清除过程中起着重要作用，因此研究了摄入乙醇对表面活性剂杀菌活性的影响。正常的由普通饲料喂养大鼠的表面活性剂表现出强大的抗肺炎球菌活性，即使是针对在有利条件下于营养丰富培养基中生长的细菌。相比之下，与由普通饲料喂养大鼠的表面活性剂相比，由乙醇喂养大鼠和热量限制对照喂养大鼠的表面活性剂抗肺炎球菌活性显著降低。乙醇摄入或热量限制所导致的表面活性剂杀菌活性降低，似乎并非通过正常表面活性剂中作为抗肺炎球菌因子的脂肪酸总量或分布的变化来介导。相反，乙醇摄入以及在较小程度上的热量限制，产生了一种游离脂肪酸的表面活性剂抑制剂，该抑制剂部分被鉴定为一种疏水蛋白。
