Lee K, Brown D, Ureña P, Ardaillou N, Ardaillou R, Deeds J, Segre G V
Department of Medicine, Massachusetts General Hospital, Boston, USA.
Am J Physiol. 1996 Jan;270(1 Pt 2):F186-91. doi: 10.1152/ajprenal.1996.270.1.F186.
In kidney, parathyroid hormone (PTH) exerts differential distal effects along the nephron. To define cells expressing receptors for PTH in kidney, we localized PTH/ PTH-related peptide (PTH/PTHrP) receptor mRNA in rat kidney by in situ hybridization. PTH/PTHrP receptor mRNA is localized to glomerular podocytes, convoluted and straight proximal tubules, the cortical portion of thick ascending limbs, and distal convoluted tubules, but was not detected in the thin limb of Henle's loop or in collecting ducts. Northern blot analysis showed that cultured human glomerular podocytes express a unique 4.0-kb PTH/PTHrP receptor transcript but do not express detectable levels of the common approximately 2.4-kb transcript found in whole kidney and in many other tissues. Whereas the tubular localization of PTH/PTHrP receptor mRNA coincides well with previously known sites of PTH action, the intense expression and the unique size of the PTH/PTHrP receptor transcript in glomerular podocytes suggest that PTH and/or PTHrP may play a role(s) in glomerular function.
在肾脏中，甲状旁腺激素（PTH）在肾单位的不同远曲部位发挥作用。为了确定肾脏中表达PTH受体的细胞，我们通过原位杂交技术在大鼠肾脏中定位了PTH/甲状旁腺激素相关肽（PTH/PTHrP）受体mRNA。PTH/PTHrP受体mRNA定位于肾小球足细胞、近曲小管的曲部和直部、髓袢升支粗段的皮质部以及远曲小管，但在髓袢细段或集合管中未检测到。Northern印迹分析表明，培养的人肾小球足细胞表达一种独特的4.0 kb PTH/PTHrP受体转录本，但不表达在全肾和许多其他组织中常见的约2.4 kb转录本的可检测水平。虽然PTH/PTHrP受体mRNA的肾小管定位与先前已知的PTH作用位点非常吻合，但肾小球足细胞中PTH/PTHrP受体转录本的强烈表达和独特大小表明，PTH和/或PTHrP可能在肾小球功能中发挥作用。
