Joseph H L, Anderson G L, Barker J H, Roisen F J, Weiner L J, Tobin G R
Department of Anatomical Sciences, University of Louisville, School of Medicine, Kentucky 40202, USA.
J Surg Res. 1996 Feb 15;61(1):197-200. doi: 10.1006/jsre.1996.0104.
The effects of locally injected combined colchicine and D-penicillamine on wound contraction were investigated in a murine model. Two full-thickness excisional wounds were made on either side of the back of hairless (hr/hr) mice. A volume of 0.15 ml of colchicine, D-penicillamine, or combined colchicine and D-penicillamine in normal saline vehicle were injected daily into the wound on one side of the animal and 0.15 ml of vehicle alone was injected into the wound on the other side for 5 or 10 days; thus, each animal served as its own control. The surface area of each wound was measured on Days 0, 5, and 10 to determine an index of the rate of wound contraction. At the end of the experimental period (Day 5 or 10), wounds were excised en bloc from euthanized animals for histological studies. The following histological parameters were determined: the thickness of the granulation tissue, the number of fibroblasts in granulation tissue per unit area, and the number of inflammatory cells (neutrophils, lymphocytes, macrophages, mast cells) in subjacent muscle per unit area. Our data showed that after 5 days of treatment, wound contraction was significantly inhibited only in wounds treated with combined colchicine and D-penicillamine. Wound contraction was significantly inhibited even after 10 days of treatment with the combination. Histological studies revealed that although the thickness of the granulation tissue and the number of inflammatory cells in subjacent muscle were decreased by D-penicillamine alone, only combined colchicine and D-penicillamine decreased the thickness of the granulation tissue, fibroblasts in granulation tissue, and inflammatory cells in subjacent muscle. Our data suggests that very low concentrations of colchicine and D-penicillamine when combined and injected locally may be potentially useful in controlling surface scar formation.
在小鼠模型中研究了局部注射秋水仙碱和D-青霉胺联合用药对伤口收缩的影响。在无毛（hr/hr）小鼠背部两侧制作两个全层切除伤口。每天向动物一侧伤口注射0.15 ml秋水仙碱、D-青霉胺或秋水仙碱与D-青霉胺的生理盐水混合液，另一侧伤口注射0.15 ml生理盐水，持续5天或10天；因此，每只动物作为自身对照。在第0、5和10天测量每个伤口的表面积，以确定伤口收缩率指标。在实验期结束时（第5天或10天），从安乐死的动物身上整块切除伤口用于组织学研究。确定以下组织学参数：肉芽组织厚度、单位面积肉芽组织中的成纤维细胞数量以及相邻肌肉中单位面积的炎症细胞（中性粒细胞、淋巴细胞、巨噬细胞、肥大细胞）数量。我们的数据显示，治疗5天后，仅秋水仙碱与D-青霉胺联合治疗的伤口收缩受到显著抑制。联合治疗10天后，伤口收缩仍受到显著抑制。组织学研究表明，虽然单独使用D-青霉胺可使肉芽组织厚度和相邻肌肉中的炎症细胞数量减少，但只有秋水仙碱与D-青霉胺联合使用可使肉芽组织厚度、肉芽组织中的成纤维细胞以及相邻肌肉中的炎症细胞减少。我们的数据表明，极低浓度的秋水仙碱和D-青霉胺联合局部注射可能对控制表面瘢痕形成有潜在作用。
