Joseph H L, Roisen F J, Anderson G L, Barker J H, Weiner L J, Tobin G R
Department of Surgery, College of Physicians and Surgeons of Columbia University (Harlem Hospital), New York, New York, USA.
Am J Surg. 1997 Sep;174(3):347-50. doi: 10.1016/s0002-9610(97)00100-1.
Previous studies using systematically administered lathyrogens to inhibit wound contractures have produced inconsistent results. The purpose of this study was to investigate the effects of lathyrogenic drugs on wound contraction when injected locally.
Two symmetrical full-thickness wounds were made on the dorsum of either side of hairless (hr/hr) mice; thus, each animal served as its own control. Animals were divided into groups receiving daily local injections of beta-aminopropionitrile or D-penicillamine, or both beta-aminopropionitrile and D-penicillamine and normal saline vehicle (control side) for 5 or 10 days. The rate of contraction was determined by serial measurements of the surface area of each wound during the treatment period. At the end of the treatment period, the wounds were excised en bloc with the chest wall and prepared for blinded histological analysis. Granulation tissue thickness, number of fibroblasts in granulation tissue per unit area, number of inflammatory cells (neutrophils, lymphocytes, macrophages and mast cells) in subjacent muscle per unit area, and collagen deposition in subjacent muscle were determined.
Wound contraction, granulation tissue thickness, and collagen deposition in subjacent muscle were decreased only in wounds treated with beta-aminopropionitrile plus D-penicillamine. Collagen deposition in subjacent muscle was also decreased in wounds treated with D-penicillamine alone. Neither drug alone nor the combination affected the number of inflammatory cells in subjacent muscle. Body weight was not affected by the experimental procedures.
The combination of beta-aminopropionitrile and D-penicillamine is potentially useful for inhibiting contracture formation when injected locally.
以往使用系统性给予致跛行物质来抑制伤口挛缩的研究结果并不一致。本研究的目的是调查致跛行药物局部注射时对伤口收缩的影响。
在无毛（hr/hr）小鼠两侧背部制造两个对称的全层伤口；因此，每只动物自身作为对照。将动物分为接受每日局部注射β-氨基丙腈或D-青霉胺，或同时注射β-氨基丙腈和D-青霉胺以及生理盐水载体（对照侧）的组，持续5天或10天。在治疗期间通过连续测量每个伤口的表面积来确定收缩率。在治疗期结束时，将伤口与胸壁整块切除并准备进行盲法组织学分析。测定肉芽组织厚度、单位面积肉芽组织中的成纤维细胞数量、下方肌肉中单位面积的炎性细胞（中性粒细胞、淋巴细胞、巨噬细胞和肥大细胞）数量以及下方肌肉中的胶原沉积。
仅在接受β-氨基丙腈加D-青霉胺治疗的伤口中，伤口收缩、肉芽组织厚度和下方肌肉中的胶原沉积减少。单独使用D-青霉胺治疗的伤口中下方肌肉的胶原沉积也减少。单独使用任何一种药物或联合使用均不影响下方肌肉中的炎性细胞数量。体重不受实验操作影响。
β-氨基丙腈和D-青霉胺联合局部注射可能对抑制挛缩形成有用。
