Hayase M, Ogawa Y, Katsuura G, Shintaku H, Hosoda K, Nakao K
Shionogi Research Laboratories, Osaka, Japan.
Am J Physiol. 1996 Aug;271(2 Pt 1):E333-9. doi: 10.1152/ajpendo.1996.271.2.E333.
To elucidate the regulation of obese (ob) gene expression in obesity and diabetes, we examined ob gene expression in KK mice and congenic lethal yellow obese KKAy mice. Northern blot analysis revealed that the ob mRNA levels are roughly equivalent in each of the epididymal, mesenteric, and subcutaneous white adipose tissue (WAT) from KK and KKAy mice at 4 wk of age, when the obese phenotype of KKAy mice was not apparent. Expression of the ob gene was augmented in the mesenteric and subcutaneous WAT but was unchanged in the epididymal WAT in KKAy mice at 12 wk of age, when KKAy mice developed marked obesity with hyperglycemia, hyperlipidemia, and hyperinsulinemia. The ob gene expression was also examined during fasting in 12-wk-old KK and KKAy mice. After 24 or 72 h of fasting in both mouse strains, ob gene expression was downregulated in the epididymal and mesenteric WAT but was unchanged in the subcutaneous WAT. The present study demonstrates that adipose tissue expression of the ob gene is regulated depending on the nutritional status in KK and KKAy mice.
为阐明肥胖和糖尿病中肥胖（ob）基因表达的调控机制，我们检测了KK小鼠和同基因致死性黄色肥胖KKAy小鼠的ob基因表达。Northern印迹分析显示，4周龄的KK和KKAy小鼠附睾、肠系膜和皮下白色脂肪组织（WAT）中的ob mRNA水平大致相当，此时KKAy小鼠的肥胖表型并不明显。12周龄时，KKAy小鼠出现明显肥胖，并伴有高血糖、高血脂和高胰岛素血症，此时其肠系膜和皮下WAT中ob基因的表达增加，而附睾WAT中ob基因的表达未发生变化。我们还检测了12周龄KK和KKAy小鼠禁食期间的ob基因表达。两种小鼠品系禁食24或72小时后，附睾和肠系膜WAT中ob基因的表达下调，而皮下WAT中ob基因的表达未发生变化。本研究表明，KK和KKAy小鼠脂肪组织中ob基因的表达受营养状态的调控。
