Morisset J, Levenez F, Corring T, Benrezzak O, Pelletier G, Calvo E
Départment de Médecine, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.
Am J Physiol. 1996 Aug;271(2 Pt 1):E397-402. doi: 10.1152/ajpendo.1996.271.2.E397.
Studies performed on pig indicated that its pancreas was insensitive to the gastrointestinal hormone cholecystokinin (CCK) and suggested that its secretions were rather under the control of the neurotransmitter acetylcholine. This study was performed to determine reasons for this insensitivity by comparing secretory responses to different secretagogues and establishing the dominant CCK receptor type. Pancreatic acini prepared from weaned piglets were evaluated for their sensitivity to carbamylcholine (Cch), caerulein, JMV-180, and secretin. RNA were extracted for CCK-A and CCK-B receptor expression using specific cRNA probes. Results indicate that pig pancreatic acini are sensitive to Cch and relatively insensitive to caerulein with no response to JMV-180, a CCKA agonist, or secretin; MK-329, a CCK-A receptor antagonist, significantly inhibited caerulein-induced enzyme secretion from 10(-8) M. The pig pancreas expresses few CCK-A mRNA receptors but a majority of CCK-B. These data demonstrate that the pig pancreas expresses a majority of CCK-B receptors. In conclusion, the pig pancreas possesses a large majority of CCK-B receptors responsible for their low sensitivity to CCK.
对猪进行的研究表明，其胰腺对胃肠激素胆囊收缩素（CCK）不敏感，并表明其分泌受神经递质乙酰胆碱的控制。进行本研究以通过比较对不同促分泌素的分泌反应并确定主要的CCK受体类型来确定这种不敏感性的原因。评估从断奶仔猪制备的胰腺腺泡对氨甲酰胆碱（Cch）、蛙皮素、JMV - 180和促胰液素的敏感性。使用特异性cRNA探针提取RNA以检测CCK - A和CCK - B受体表达。结果表明，猪胰腺腺泡对Cch敏感，对蛙皮素相对不敏感，对CCKA激动剂JMV - 180或促胰液素无反应；CCK - A受体拮抗剂MK - 329在10(-8) M时显著抑制蛙皮素诱导的酶分泌。猪胰腺表达很少的CCK - A mRNA受体，但大部分是CCK - B。这些数据表明猪胰腺表达大部分CCK - B受体。总之，猪胰腺拥有绝大多数CCK - B受体，这导致其对CCK敏感性较低。
