Wilson M, Marcuz A, du Pasquier L
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Dev Immunol. 1995;4(3):227-34. doi: 10.1155/1995/38639.
The tadpole B-cell repertoire is less diverse than that of the adult frog; their antibodies are of lower affinity and are less heterogenous. In order to determine whether this difference is due to a lack of or a reduced rate of somatic hypermutation, we analyzed and compared cDNA sequences utilizing VH1 elements with germline counterparts in isogenic LG7 tadpoles during an immune response. Indeed, tadpole VH1 sequences contained somatic mutations. There were zero to 5 mutations per sequence, all single base-point mutations, with the high ratio of GC to AT base-pair alterations similar to that observed in adult frogs.
蝌蚪的B细胞库比成年青蛙的多样性更低；它们的抗体亲和力较低且异质性较小。为了确定这种差异是由于体细胞超突变的缺乏还是速率降低，我们在免疫反应期间分析并比较了同基因LG7蝌蚪中利用VH1元件的cDNA序列与其种系对应序列。实际上，蝌蚪的VH1序列包含体细胞突变。每个序列有0至5个突变，均为单碱基点突变，GC与AT碱基对改变的高比例与成年青蛙中观察到的相似。
