Dickson B J, van der Straten A, Dominguez M, Hafen E
Zoologisches Institut, Universität Zũrich, Switzerland.
Genetics. 1996 Jan;142(1):163-71. doi: 10.1093/genetics/142.1.163.
The R7 fate is specified during Drosophila eye development by an inductive signal transduced intracellularly via the Raf kinase. We have performed a genetic screen for dominant mutations that alter the efficiency with which cells respond to a constitutively activated Raf kinase. Such mutations may affect genes involved in signal transduction downstream of Raf. We have isolated 44 mutations that define eight genes. One of these encodes a mitogen-activated protein kinase homologue: another is a putative target gene of this signaling pathway. We present the results of this screen in detail, as well as a preliminary genetic analysis of the six loci still to be characterized molecularly.
在果蝇眼睛发育过程中，R7细胞命运是由通过Raf激酶在细胞内转导的诱导信号所决定的。我们针对显性突变进行了一项遗传筛选，这些突变会改变细胞对组成型激活的Raf激酶作出反应的效率。此类突变可能影响Raf下游信号转导中涉及的基因。我们分离出了44个突变，这些突变定义了8个基因。其中一个基因编码一种丝裂原活化蛋白激酶同源物：另一个是该信号通路的一个假定靶基因。我们详细展示了该筛选的结果，以及对仍有待进行分子特征鉴定的6个基因座的初步遗传分析。
