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1
The cytosolic subunit p67phox contains an NADPH-binding site that participates in catalysis by the leukocyte NADPH oxidase.胞质亚基p67phox含有一个NADPH结合位点，该位点参与白细胞NADPH氧化酶的催化作用。
J Clin Invest. 1996 Aug 15;98(4):977-83. doi: 10.1172/JCI118882.
2
The cytosolic subunit p67phox of the NADPH-oxidase complex does not bind NADPH.NADPH氧化酶复合物的胞质亚基p67phox不结合NADPH。
FEBS Lett. 2009 Oct 6;583(19):3225-9. doi: 10.1016/j.febslet.2009.09.011. Epub 2009 Sep 12.
3
NADPH dehydrogenase activity of p67PHOX, a cytosolic subunit of the leukocyte NADPH oxidase.白细胞NADPH氧化酶的胞质亚基p67PHOX的NADPH脱氢酶活性。
Biochemistry. 1999 May 4;38(18):5746-53. doi: 10.1021/bi982750f.
4
Use of an affinity label to probe the function of the NADPH binding component of the respiratory burst oxidase of human neutrophils.使用亲和标记物探究人类中性粒细胞呼吸爆发氧化酶的NADPH结合成分的功能。
J Biol Chem. 1989 Jul 25;264(21):12243-8.
5
Nucleoside-triphosphate binding of the two cytosolic components of the respiratory burst oxidase system: evidence for its inhibition by the 2',3'-dialdehyde derivative of NADPH and desensitization in their translocated states.呼吸爆发氧化酶系统两种胞质成分的核苷三磷酸结合：NADPH的2',3'-二醛衍生物对其抑制作用及在其转位状态下脱敏的证据。
Biochim Biophys Acta. 1993 Dec 16;1220(1):21-30. doi: 10.1016/0167-4889(93)90092-4.
6
Identification of the NADPH-binding protein of the neutrophil superoxide-generating oxidase of guinea pigs.豚鼠中性粒细胞超氧化物生成氧化酶的NADPH结合蛋白的鉴定
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7
Interactions between the cytosolic components p47phox and p67phox of the human neutrophil NADPH oxidase that are not required for activation in the cell-free system.人中性粒细胞NADPH氧化酶的胞质成分p47phox和p67phox之间的相互作用，在无细胞系统中激活时并非必需。
J Biol Chem. 1995 May 12;270(19):11216-21. doi: 10.1074/jbc.270.19.11216.
8
Mechanisms of NADPH oxidase activation: translocation of p40phox, Rac1 and Rac2 from the cytosol to the membranes in human neutrophils lacking p47phox or p67phox.NADPH氧化酶激活机制：在缺乏p47phox或p67phox的人类中性粒细胞中，p40phox、Rac1和Rac2从胞质溶胶向细胞膜的转位。
Biochem J. 1996 Mar 1;314 ( Pt 2)(Pt 2):409-12. doi: 10.1042/bj3140409.
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Cytosolic guanine nucleotide-binding protein Rac2 operates in vivo as a component of the neutrophil respiratory burst oxidase. Transfer of Rac2 and the cytosolic oxidase components p47phox and p67phox to the submembranous actin cytoskeleton during oxidase activation.胞质鸟嘌呤核苷酸结合蛋白Rac2在体内作为中性粒细胞呼吸爆发氧化酶的一个组分发挥作用。在氧化酶激活过程中，Rac2以及胞质氧化酶组分p47phox和p67phox转移至膜下肌动蛋白细胞骨架。
J Biol Chem. 1994 Mar 4;269(9):6729-34.
10
Affinity labeling of the cytosolic and membrane components of the respiratory burst oxidase by the 2',3'-dialdehyde derivative of NADPH. Evidence for a cytosolic location of the nucleotide-binding site in the resting cell.NADPH的2',3'-二醛衍生物对呼吸爆发氧化酶的胞质和膜成分进行亲和标记。静息细胞中核苷酸结合位点位于胞质的证据。
J Biol Chem. 1989 Feb 5;264(4):1958-62.

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NADPH oxidases: an overview from structure to innate immunity-associated pathologies.烟酰胺腺嘌呤二核苷酸磷酸氧化酶：从结构到与先天免疫相关疾病的概述
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The inflammatory NADPH oxidase enzyme modulates motor neuron degeneration in amyotrophic lateral sclerosis mice.炎症性烟酰胺腺嘌呤二核苷酸磷酸氧化酶调节肌萎缩侧索硬化症小鼠的运动神经元变性。
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12132-7. doi: 10.1073/pnas.0603670103. Epub 2006 Jul 28.
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Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II.中性粒细胞呼吸爆发氧化酶的组装：p67PHOX 与细胞色素 b558 II 之间的直接相互作用。
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4262-5. doi: 10.1073/pnas.072345299. Epub 2002 Mar 26.
5
Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558.中性粒细胞呼吸爆发氧化酶的组装：p67PHOX与细胞色素b558之间的直接相互作用。
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3001-5. doi: 10.1073/pnas.061029698.
6
Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase.Gp91（phox）是产生超氧化物的NADPH氧化酶的血红素结合亚基。
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7993-8. doi: 10.1073/pnas.95.14.7993.
7
The molecular basis of chronic granulomatous disease.慢性肉芽肿病的分子基础。
Springer Semin Immunopathol. 1998;19(4):417-34. doi: 10.1007/BF00792600.

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A syndrome of recurrent infection and infiltration of viscera by pigmented lipid histiocytes.一种色素性脂质组织细胞反复感染并浸润内脏的综合征。
Pediatrics. 1957 Sep;20(3):431-8.
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A fatal granulomatosus of childhood: the clinical study of a new syndrome.一种儿童期致命性肉芽肿病：一种新综合征的临床研究。
Minn Med. 1957 May;40(5):309-12.
3
Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase components.Rac的易位与NADPH氧化酶激活相关。氧化酶组分等摩尔易位的证据。
J Biol Chem. 1993 Oct 5;268(28):20983-7.
4
Generation of superoxide by purified and relipidated cytochrome b559 in the absence of cytosolic activators.在没有胞质激活剂的情况下，纯化并重新脂质化的细胞色素b559产生超氧化物。
FEBS Lett. 1993 Jul 19;327(1):57-62. doi: 10.1016/0014-5793(93)81039-3.
5
The respiratory burst oxidase of human neutrophils. Guanine nucleotides and arachidonate regulate the assembly of a multicomponent complex in a semirecombinant cell-free system.人类中性粒细胞的呼吸爆发氧化酶。鸟嘌呤核苷酸和花生四烯酸在半重组无细胞系统中调节多组分复合物的组装。
J Biol Chem. 1993 Apr 25;268(12):8624-31.
6
Superoxide production by cytochrome b559. Mechanism of cytosol-independent activation.细胞色素b559产生超氧化物。非胞质溶胶依赖性激活机制。
FEBS Lett. 1994 Feb 7;338(3):285-9. doi: 10.1016/0014-5793(94)80285-8.
7
The requirement of p47 phosphorylation for activation of NADPH oxidase by opsonized zymosan in human neutrophils.人中性粒细胞中调理酵母聚糖激活NADPH氧化酶对p47磷酸化的需求。
Biochim Biophys Acta. 1994 Feb 17;1220(3):253-60. doi: 10.1016/0167-4889(94)90146-5.
8
NADPH-binding component of the respiratory burst oxidase system: studies using neutrophil membranes from patients with chronic granulomatous disease lacking the beta-subunit of cytochrome b558.呼吸爆发氧化酶系统的NADPH结合成分：使用来自缺乏细胞色素b558β亚基的慢性肉芽肿病患者中性粒细胞膜的研究
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9
Nucleoside-triphosphate binding of the two cytosolic components of the respiratory burst oxidase system: evidence for its inhibition by the 2',3'-dialdehyde derivative of NADPH and desensitization in their translocated states.呼吸爆发氧化酶系统两种胞质成分的核苷三磷酸结合：NADPH的2',3'-二醛衍生物对其抑制作用及在其转位状态下脱敏的证据。
Biochim Biophys Acta. 1993 Dec 16;1220(1):21-30. doi: 10.1016/0167-4889(93)90092-4.
10
Isolation of a complex of respiratory burst oxidase components from resting neutrophil cytosol.从静息中性粒细胞胞质溶胶中分离呼吸爆发氧化酶成分复合物。
Biochemistry. 1994 Mar 15;33(10):2907-11. doi: 10.1021/bi00176a021.

胞质亚基p67phox含有一个NADPH结合位点，该位点参与白细胞NADPH氧化酶的催化作用。

The cytosolic subunit p67phox contains an NADPH-binding site that participates in catalysis by the leukocyte NADPH oxidase.

作者信息

Smith R M, Connor J A, Chen L M, Babior B M

机构信息

The Department of Medicine, University of California, San Diego 92093, USA.

出版信息

J Clin Invest. 1996 Aug 15;98(4):977-83. doi: 10.1172/JCI118882.

DOI:10.1172/JCI118882
PMID:8770870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC507513/
Abstract

The NADPH-dependent respiratory burst oxidase of human neutrophils catalyzes the reduction of oxygen to superoxide using NADPH as the electron donor and is essential for normal host defenses. To gain insight into the function of the various oxidase subunits that are required for the full expression of catalytic activity, we studied the interactions between the 2',3'-dialdehyde derivative of NADPH (NADPH dialdehyde) and neutrophil cytosol. NADPH dialdehyde treatment of cytosol resulted in the loss of the ability of the cytosol to participate in cell-free oxidase activation; this inactivation was blocked by NADPH but not by NAD, NADP, or GTP. Partial purification of neutrophil cytosol yielded a single peak which could restore the activity lost in cytosol treated with NADPH dialdehyde. This peak contained p67phox but not p47phox or Rac2. Purified recombinant p67phox was similarly able to restore the activity lost in NADPH dialdehyde-treated cytosol and bound [32P]NADPH dialdehyde in a specific fashion. The activity of recombinant p67phox in cell-free oxidase assays was lost on treatment with NADPH dialdehyde. Together, these data suggest p67phox contains the catalytic NADPH-binding site of the leukocyte NADPH oxidase.

摘要

人类中性粒细胞中依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的呼吸爆发氧化酶以NADPH作为电子供体催化氧气还原为超氧化物，这对于正常的宿主防御至关重要。为了深入了解催化活性充分表达所需的各种氧化酶亚基的功能，我们研究了NADPH的2',3'-二醛衍生物（NADPH二醛）与中性粒细胞胞质溶胶之间的相互作用。用NADPH二醛处理胞质溶胶导致其参与无细胞氧化酶激活的能力丧失；这种失活被NADPH阻断，但不被NAD、NADP或鸟苷三磷酸（GTP）阻断。对中性粒细胞胞质溶胶进行部分纯化得到一个单一峰，该峰可以恢复用NADPH二醛处理的胞质溶胶中丧失的活性。这个峰含有p67phox，但不含有p47phox或Rac2。纯化的重组p67phox同样能够恢复在NADPH二醛处理的胞质溶胶中丧失的活性，并以特定方式结合[32P]NADPH二醛。在无细胞氧化酶测定中，重组p67phox的活性在用NADPH二醛处理后丧失。总之，这些数据表明p67phox包含白细胞NADPH氧化酶的催化性NADPH结合位点。