大肠杆菌外膜蛋白A的另一种拓扑模型。
An alternative topological model for Escherichia coli OmpA.
作者信息
Stathopoulos C
机构信息
Department of Microbiology, University of Texas, Austin 78712, USA.
出版信息
Protein Sci. 1996 Jan;5(1):170-3. doi: 10.1002/pro.5560050122.
Abstract
The current topological model for the Escherichia coli outer membrane protein OmpA predicts eight N-terminal transmembrane segments followed by a long periplasmic tail. Several recent reports have raised serious doubts about the accuracy of this prediction. An alternative OmpA model has been constructed using (1) computer-aided predictions developed specifically to predict topology of bacterial outer membrane porins, (2) the results of two reports that identified sequence homologies between OmpA and other peptidoglycan-associated proteins, and (3) biochemical, immunochemical, and genetic topological data on proteins of the OmpA family provided by numerous previous studies. The new model not only agrees with the varied experimental data concerning OmpA but also provides an improved understanding of the relationship between the structure and the multifunctional role of OmpA in the bacterial outer membrane.
摘要
目前关于大肠杆菌外膜蛋白OmpA的拓扑模型预测其有八个N端跨膜片段，随后是一条长的周质尾巴。最近的几份报告对这一预测的准确性提出了严重质疑。已构建了一种替代的OmpA模型，该模型使用了：（1）专门为预测细菌外膜孔蛋白拓扑结构而开发的计算机辅助预测方法；（2）两份报告的结果，这两份报告确定了OmpA与其他肽聚糖相关蛋白之间的序列同源性；（3）众多先前研究提供的关于OmpA家族蛋白的生化、免疫化学和遗传拓扑数据。新模型不仅与关于OmpA的各种实验数据相符，还增进了人们对OmpA在细菌外膜中的结构与多功能作用之间关系的理解。
相似文献
1
An alternative topological model for Escherichia coli OmpA.大肠杆菌外膜蛋白A的另一种拓扑模型。
Protein Sci. 1996 Jan;5(1):170-3. doi: 10.1002/pro.5560050122.
2
Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp. A site-directed fluorescence study.外膜蛋白A与周质伴侣蛋白Skp形成复合物时的结合区域。一项定点荧光研究。
Biochemistry. 2009 Jun 9;48(22):4926-36. doi: 10.1021/bi9004039.
3
In vivo membrane assembly of split variants of the E.coli outer membrane protein OmpA.大肠杆菌外膜蛋白OmpA裂解变体的体内膜组装
EMBO J. 1996 Jul 15;15(14):3529-37.
4
Structure of the outer membrane protein A transmembrane domain.外膜蛋白A跨膜结构域的结构。
Nat Struct Biol. 1998 Nov;5(11):1013-7. doi: 10.1038/2983.
5
The function of OmpA in Escherichia coli.大肠杆菌中OmpA的功能。
Biochem Biophys Res Commun. 2002 Mar 29;292(2):396-401. doi: 10.1006/bbrc.2002.6657.
6
Membrane assembly of the Escherichia coli outer membrane protein OmpA: exploring sequence constraints on transmembrane beta-strands.大肠杆菌外膜蛋白OmpA的膜组装：探索跨膜β链上的序列限制
J Mol Biol. 1999 Jan 29;285(4):1801-10. doi: 10.1006/jmbi.1998.2405.
7
A molecular Swiss army knife: OmpA structure, function and expression.一种分子瑞士军刀：外膜蛋白A的结构、功能与表达
FEMS Microbiol Lett. 2007 Aug;273(1):1-11. doi: 10.1111/j.1574-6968.2007.00778.x. Epub 2007 Jun 6.
8
Infrared dichroism of twisted beta-sheet barrels. The structure of E. coli outer membrane proteins.扭曲β-折叠桶的红外二色性。大肠杆菌外膜蛋白的结构。
J Mol Biol. 2000 Mar 31;297(3):803-8. doi: 10.1006/jmbi.2000.3557.
9
Export of altered forms of an Escherichia coli K-12 outer membrane protein (OmpA) can inhibit synthesis of unrelated outer membrane proteins.大肠杆菌K-12外膜蛋白（OmpA）变异形式的输出可抑制不相关外膜蛋白的合成。
J Mol Biol. 1990 Nov 5;216(1):39-47. doi: 10.1016/S0022-2836(05)80059-2.
10
Membrane assembly of circularly permuted variants of the E. coli outer membrane protein OmpA.
J Mol Biol. 1995 Jul 28;250(5):617-26. doi: 10.1006/jmbi.1995.0403.
引用本文的文献
1
Rapid detection and capture of clinical Escherichia coli strains mediated by OmpA-targeting nanobodies.由靶向OmpA的纳米抗体介导的临床大肠杆菌菌株的快速检测与捕获
Commun Biol. 2025 Jul 14;8(1):1047. doi: 10.1038/s42003-025-08345-9.
2
Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution.大肠杆菌外膜蛋白 A 的等位基因变异：对细胞表面特性、应激耐受性和等位基因分布的影响。
PLoS One. 2022 Oct 13;17(10):e0276046. doi: 10.1371/journal.pone.0276046. eCollection 2022.
3
β-Barrels and Amyloids: Structural Transitions, Biological Functions, and Pathogenesis.β-桶状结构和淀粉样纤维：结构转变、生物学功能与发病机制。
Int J Mol Sci. 2021 Oct 20;22(21):11316. doi: 10.3390/ijms222111316.
4
Defining the function of OmpA in the Rcs stress response.定义 OmpA 在 Rcs 应激反应中的功能。
Elife. 2020 Sep 28;9:e60861. doi: 10.7554/eLife.60861.
5
How the assembly and protection of the bacterial cell envelope depend on cysteine residues.细菌细胞包膜的组装和保护如何依赖半胱氨酸残基。
J Biol Chem. 2020 Aug 21;295(34):11984-11994. doi: 10.1074/jbc.REV120.011201. Epub 2020 Jun 2.
6
Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins.改造大肠杆菌细胞表面以开发一种重组蛋白的一步纯化方法。
AMB Express. 2018 Jun 30;8(1):107. doi: 10.1186/s13568-018-0638-8.
7
Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi.对鼠伤寒沙门氏菌和伯氏疏螺旋体OmpA C末端结构域的PG结合、构象变化和二聚化的见解。
Protein Sci. 2017 Sep;26(9):1738-1748. doi: 10.1002/pro.3209. Epub 2017 Jun 19.
8
The role of short-chain conjugated poly-(R)-3-hydroxybutyrate (cPHB) in protein folding.短链共轭聚（R）-3-羟基丁酸酯（cPHB）在蛋白质折叠中的作用。
Int J Mol Sci. 2013 May 23;14(6):10727-48. doi: 10.3390/ijms140610727.
9
In vivo protein complex topologies: sights through a cross-linking lens.体内蛋白质复合物拓扑结构：交联视角下的观察。
Proteomics. 2012 May;12(10):1565-75. doi: 10.1002/pmic.201100516.
10
Insights into the structure and assembly of Escherichia coli outer membrane protein A.大肠杆菌外膜蛋白 A 的结构与组装的研究进展
FEBS J. 2012 Mar;279(6):894-909. doi: 10.1111/j.1742-4658.2012.08484.x. Epub 2012 Feb 10.
本文引用的文献
1
Proposal for a peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins.关于某些细菌细胞表面蛋白C末端区域中肽聚糖结合α-螺旋基序的提议。
Mol Microbiol. 1995 Jun;16(6):1269-70. doi: 10.1111/j.1365-2958.1995.tb02348.x.
2
Insertion derivatives containing segments of up to 16 amino acids identify surface- and periplasm-exposed regions of the FhuA outer membrane receptor of Escherichia coli K-12.包含长达16个氨基酸片段的插入衍生物可识别大肠杆菌K-12的FhuA外膜受体的表面暴露区域和周质暴露区域。
J Bacteriol. 1993 Feb;175(3):826-39. doi: 10.1128/jb.175.3.826-839.1993.
3
Prediction of membrane-spanning beta-strands and its application to maltoporin.跨膜β链的预测及其在麦芽糖孔蛋白中的应用。
Protein Sci. 1993 Aug;2(8):1361-3. doi: 10.1002/pro.5560020820.
4
Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface.在细胞外表面表达功能性抗体片段的大肠杆菌的生产及荧光激活细胞分选
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10444-8. doi: 10.1073/pnas.90.22.10444.
5
Membrane topology and assembly of the outer membrane protein OmpA of Escherichia coli K12.大肠杆菌K12外膜蛋白OmpA的膜拓扑结构与组装
Mol Gen Genet. 1994 Apr;243(2):127-35. doi: 10.1007/BF00280309.
6
The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan.OmpA相关外膜蛋白与MotB之间的C末端序列保守性表明，在革兰氏阳性菌和革兰氏阴性菌中存在共同功能，可能在于这些结构域与肽聚糖的相互作用。
Mol Microbiol. 1994 Apr;12(2):333-4. doi: 10.1111/j.1365-2958.1994.tb01021.x.
7
OmpA-FMDV VP1 fusion proteins: production, cell surface exposure and immune responses to the major antigenic domain of foot-and-mouth disease virus.外膜蛋白A-口蹄疫病毒VP1融合蛋白：生产、细胞表面暴露及对口蹄疫病毒主要抗原结构域的免疫反应
Vaccine. 1994 May;12(6):492-8. doi: 10.1016/0264-410x(94)90305-0.
8
Linker-insertion mutagenesis of Pseudomonas aeruginosa outer membrane protein OprF.铜绿假单胞菌外膜蛋白OprF的接头插入诱变
Mol Microbiol. 1993 Oct;10(2):283-92.
9
Characterization of two membrane-bound forms of OmpA.两种膜结合形式的外膜蛋白A的特性分析。
Biochemistry. 1995 Feb 14;34(6):1921-9. doi: 10.1021/bi00006a013.
10
From sequence alignment to structure prediction: the case of the OmpF porin family.从序列比对到结构预测：外膜孔蛋白F家族的实例
Mol Microbiol. 1994 Oct;14(1):188-9. doi: 10.1111/j.1365-2958.1994.tb01279.x.
Zotero 插件