阿尔茨海默病女性患者血小板中5-羟色胺摄取及其动力学

Serotonin uptake and its kinetics in platelets of women with Alzheimer's disease.

作者信息

Kumar A M, Kumar M, Sevush S, Ruiz J, Eisdorfer C

机构信息

Department of Psychiatry and Behavioral Sciences, University of Miami School of Medicine, FL 33101, USA.

出版信息

Psychiatry Res. 1995 Nov 29;59(1-2):145-50. doi: 10.1016/0165-1781(95)02786-6.

DOI:10.1016/0165-1781(95)02786-6

PMID:8771229

Abstract

The uptake of serotonin (14C-5-hydroxytryptamine, 5HT) in platelets and its kinetic characteristics were investigated in a group of women (n = 20) with probable Alzheimer's Disease (mean age = 76.0, years, SD = 8.27, range = 63-88) and in healthy normal women (n = 18, mean age = 72.6 years, SD = 7.24, range = 61-84). Both the apparent affinity of binding of 5HT to the platelet membrane (Km) and the maximum velocity (Vmax) of the rate of transport of 14C-5HT into platelets were significantly higher in the Alzheimer's Disease group than in the normal comparison group. Initial velocity of 14C-5HT uptake not passive diffusion (nonspecific uptake of 14C-5HT at 4 degrees C) differed significantly in the two groups. Our findings suggest abnormalities in the kinetic mechanisms involved in the uptake of 14C-5HT by platelets in women with Alzheimer's Disease.

摘要

在一组可能患有阿尔茨海默病的女性（n = 20，平均年龄 = 76.0岁，标准差 = 8.27，范围 = 63 - 88）和健康正常女性（n = 18，平均年龄 = 72.6岁，标准差 = 7.24，范围 = 61 - 84）中，研究了血小板中血清素（14C - 5 - 羟色胺，5HT）的摄取及其动力学特征。与正常对照组相比，阿尔茨海默病组中5HT与血小板膜结合的表观亲和力（Km）以及14C - 5HT转运至血小板的速率的最大速度（Vmax）均显著更高。两组中14C - 5HT摄取的初始速度而非被动扩散（4℃时14C - 5HT的非特异性摄取）存在显著差异。我们的研究结果表明，患有阿尔茨海默病的女性血小板摄取14C - 5HT所涉及的动力学机制存在异常。