Hartmann A, Azérad J, Boucher Y
Laboratoire de Physiologie de la Manducation, Paris, France.
Arch Oral Biol. 1996 Apr;41(4):333-9. doi: 10.1016/0003-9969(95)00133-6.
The increasing number of experiments using laser Doppler flowmetry in man for pulpal blood-flow recordings leads to questioning of the experimental recording conditions. The present study focused on three points: the design of the laser probe holder, the isolation of the tooth, and the influence of the recording site. A rigid polyurethane splint used in addition to different isolation devices (cotton roll, metal shield, rubber dam) was compared with a silicone splint. The silicone resulted in significantly higher values (+341%) than the polyurethane splint. The combination of the polyurethane splint with isolation devices decreased, in all cases, the flux values. The polyurethane/rubber-dam combination was the most efficient in individualizing the pulpal blood flow (-69% decrease). Recordings on non-vital teeth confirmed the hypothesis that there was periodontal contamination of the recorded flow, as the signal was abolished when using the polyurethane/rubber-dam combination. Cervical recording sites gave significantly higher values than occlusal sites (+42%). It is concluded that, in man, the part played by the periodontium may have been underestimated in previous recordings of pulpal blood flow. The use of a rubber dam in combination with a rigid splint to enhance the validity of recordings is proposed.
在人体中使用激光多普勒血流仪进行牙髓血流记录的实验数量不断增加，这引发了对实验记录条件的质疑。本研究聚焦于三点：激光探头固定器的设计、牙齿的隔离以及记录部位的影响。将一种刚性聚氨酯夹板与不同的隔离装置（棉卷、金属护罩、橡胶障）配合使用，并与硅胶夹板进行比较。结果显示，硅胶夹板产生的值显著高于聚氨酯夹板（高出341%）。在所有情况下，聚氨酯夹板与隔离装置结合使用都会降低通量值。聚氨酯/橡胶障组合在区分牙髓血流方面效率最高（降低69%）。对无活力牙的记录证实了这样的假设，即记录的血流存在牙周污染，因为使用聚氨酯/橡胶障组合时信号消失了。颈部记录部位的值显著高于咬合部位（高出42%）。得出的结论是，在人体中，牙周组织在以往牙髓血流记录中所起的作用可能被低估了。建议使用橡胶障与刚性夹板相结合的方法来提高记录的有效性。
