Takanosu M, Amasaki H, Matsumoto S, Kimata K
Department of Veterinary Anatomy, Nippon Veterinary and Animal Science University, Tokyo, Japan.
J Anat. 1996 Aug;189 ( Pt 1)(Pt 1):109-15.
The expression of proteoglycans detected by the monoclonal antibodies MO-225, 3B3 and 2B6 and of peanut agglutinin-binding molecules was examined histochemically during the development of bovine palatine ridge (PR) rudiments in fetuses with crown-rump lengths of 4-60 cm. The bovine PR rudiment has 2 characteristic developmental stages: the 1st is the positioning of the epithelial placode (EP) to the predetermined site of PR rudiment formation and the 2nd is the reorientation of the apical edge of PR rudiments to form wave-like patterns from mouth to pharynx. During the 1st stage, chondroitin 6-sulphate (C6S) was expressed strongly at the epithelial basement membrane just beneath the EP. During the 2nd stage, both C6S and disaccharide unit of Glc2sulphate-GalNAc6sulphate (GlcAsSO4-GalNAc6SO4) were distributed at the basement membrane and the mesenchyme just beneath the steeper wall of the PR rudiment. Peanut agglutinin-binding molecules were also detected in the mesenchyme, their distribution being similar to that for C6S and GlcA2SO4-GalNAc6SO4, and additionally in the epithelial cells after formation of the wave-like PR. The distribution of dermatan sulphate was not directly related to the developmental changes of PR rudiments, but it was detected in the lower mesenchymal layer which supported the protruding site of the PR rudiment after the 2nd stage. The results suggest that 2 different types of proteoglycan may be involved in the critical periods during the morphogenesis of the bovine PR rudiments.
利用单克隆抗体MO - 225、3B3和2B6以及花生凝集素结合分子，通过组织化学方法检测了顶臀长4 - 60厘米胎儿牛腭嵴（PR）原基发育过程中蛋白聚糖的表达情况。牛PR原基有两个特征性发育阶段：第一个阶段是上皮基板（EP）定位到PR原基形成的预定部位，第二个阶段是PR原基顶端边缘重新定向，从口腔到咽部形成波浪状模式。在第一阶段，硫酸软骨素6 - 硫酸酯（C6S）在上皮基板下方的上皮基底膜处强烈表达。在第二阶段，C6S和硫酸葡萄糖醛酸 - N - 乙酰半乳糖胺6 - 硫酸酯（GlcAsSO4 - GalNAc6SO4）的二糖单位都分布在PR原基较陡壁下方的基底膜和间充质中。花生凝集素结合分子也在间充质中被检测到，其分布与C6S和GlcA2SO4 - GalNAc6SO4相似，并且在波浪状PR形成后，在上皮细胞中也有检测到。硫酸皮肤素的分布与PR原基的发育变化没有直接关系，但在第二阶段后，在支持PR原基突出部位的下方间充质层中检测到了硫酸皮肤素。结果表明，两种不同类型的蛋白聚糖可能参与了牛PR原基形态发生的关键时期。
