Tanoue M, Yamaga M, Ide J, Takagi K
Department of Orthopaedic Surgery, Kumamoto University School of Medicine, Japan.
J Hand Surg Br. 1996 Jun;21(3):358-63. doi: 10.1016/s0266-7681(05)80203-7.
The conjugation of horseradish peroxidase with wheat germ agglutinin was used to identify the effect on retrograde axonal transport of stretching the rat sciatic nerve indirectly by 10% and 20% femoral lengthening with a unilateral external fixator. To investigate the relationship between retrograde axonal transport and blood flow in the stretched nerve, nerve blood flow in the sciatic nerve was measured by a hydrogen washout technique. At 11% strain (20% femoral lengthening), the numbers of horseradish peroxidase-labelled motor neuron cells and nerve blood flow had decreased by 43% and 50%, respectively. Histological examination demonstrated ischaemic changes, but not mechanical damage. However, at 6% strain (10% femoral lengthening) there were no significant abnormalities. These findings suggest that the inhibition of retrograde axonal transport can be induced by acute stretching of the peripheral nerve and that circulatory disturbance is the main cause of the inhibition of retrograde axonal transport at the low strain.
辣根过氧化物酶与麦胚凝集素的结合物被用于确定通过单侧外固定器使大鼠股骨间接延长10%和20%来拉伸坐骨神经对逆行轴突运输的影响。为了研究拉伸神经中逆行轴突运输与血流之间的关系，采用氢洗脱技术测量坐骨神经中的神经血流。在11%应变（股骨延长20%）时，辣根过氧化物酶标记的运动神经元细胞数量和神经血流分别减少了43%和50%。组织学检查显示有缺血性改变，但无机械损伤。然而，在6%应变（股骨延长10%）时，未发现明显异常。这些发现表明，外周神经的急性拉伸可诱导逆行轴突运输的抑制，并且在低应变时循环障碍是逆行轴突运输抑制的主要原因。
