Fujii T, Yoshimoto H, Nagasawa N, Bogaki T, Tamai Y, Hamachi M
Central Laboratories for Key Technology, Kirin Brewery Co. Ltd, Kanagawa, Japan.
Yeast. 1996 May;12(6):593-8. doi: 10.1002/(SICI)1097-0061(199605)12:6%3C593::AID-YEA593%3E3.0.CO;2-B.
The nucleotide sequences of alcohol acetyltransferase genes isolated from lager brewing yeast, Saccharomyces carlsbergensis have been determined. S. carlsbergensis has one ATF1 gene and another homologous gene, the Lg-ATF1 gene. There was a high degree of homology between the amino acid sequences deduced for the ATF1 protein and the Lg-ATF1 protein (75.7%), but the N-terminal region has a relatively low degree of homology. Southern analysis and contour-clamped homogeneous electric field analysis of Saccharomyces strains suggest that the ATF1 gene is located on chromosome XV in S. cerevisiae and that the Lg-ATF1 gene might originate from the "non-S. cerevisiae' genome of S. carlsbergensis, which is similar to that of S. bayanus and S. pastorianus.
已测定从贮藏啤酒酵母（卡尔斯伯酵母）中分离出的乙醇乙酰转移酶基因的核苷酸序列。卡尔斯伯酵母有一个ATF1基因和另一个同源基因，即Lg - ATF1基因。推导的ATF1蛋白和Lg - ATF1蛋白的氨基酸序列之间存在高度同源性（75.7%），但N端区域的同源性相对较低。对酵母菌株的Southern分析和轮廓夹钳均匀电场分析表明，ATF1基因位于酿酒酵母的第十五号染色体上，而Lg - ATF1基因可能起源于卡尔斯伯酵母的“非酿酒酵母”基因组，该基因组与贝酵母和巴斯德酵母的基因组相似。
