Berry A
Genencor International, Palo Alto, CA 94304-1013, USA.
Trends Biotechnol. 1996 Jul;14(7):250-6. doi: 10.1016/0167-7799(96)10033-0.
The efficiency of conversion of raw material to product is a major factor controlling the commercial viability of large-scale fermentation processes for the production of metabolites. Traditional approaches to developing microbial strains for metabolite overproduction have relied on amplifying only the enzymatic steps within the specific biosynthetic pathway of a desired product. While this approach has generally been successful for reaching high product concentrations and yields, further improvements (and hence reduced manufacturing cost) can only be achieved by redirecting carbon flux from central metabolism to the product-forming pathway. Such manipulation of central metabolic pathways promises to deliver a new generation of metabolite-producing microorganisms.
将原材料转化为产品的效率是控制大规模发酵生产代谢物过程商业可行性的一个主要因素。传统的开发用于过量生产代谢物的微生物菌株的方法仅依赖于扩增所需产品特定生物合成途径中的酶促步骤。虽然这种方法通常在达到高产品浓度和产量方面取得了成功，但只有通过将碳通量从中心代谢重新导向产物形成途径，才能实现进一步的改进（从而降低制造成本）。对中心代谢途径的这种操纵有望产生新一代生产代谢物的微生物。
