Wendisch Volker F, Bott Michael, Eikmanns Bernhard J
Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Münster, 48149 Münster, Germany.
Curr Opin Microbiol. 2006 Jun;9(3):268-74. doi: 10.1016/j.mib.2006.03.001. Epub 2006 Apr 17.
Industrial microorganisms have been developed as biocatalysts to provide new or to optimize existing processes for the biotechnological production of chemicals from renewable plant biomass. Rational strain development by metabolic engineering is crucial to successful processes, and is based on efficient genetic tools and detailed knowledge of metabolic pathways and their regulation. This review summarizes recent advances in metabolic engineering of the industrial model bacteria Escherichia coli and Corynebacterium glutamicum that led to efficient recombinant biocatalysts for the production of acetate, pyruvate, ethanol, d- and l-lactate, succinate, l-lysine and l-serine.
工业微生物已被开发为生物催化剂，用于提供新的工艺或优化现有工艺，以便从可再生植物生物质中通过生物技术生产化学品。通过代谢工程进行合理的菌株开发对于成功的工艺至关重要，并且基于高效的遗传工具以及对代谢途径及其调控的详细了解。本综述总结了工业模式细菌大肠杆菌和谷氨酸棒杆菌代谢工程的最新进展，这些进展产生了用于生产乙酸盐、丙酮酸、乙醇、d-和l-乳酸、琥珀酸盐、l-赖氨酸和l-丝氨酸的高效重组生物催化剂。
