Kathiresan A, Reid D M, Chinnappa C C
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Planta. 1996;199(3):329-35. doi: 10.1007/BF00195723.
Stem and leaf tissues of Stellaria longipes Goldie (prairie ecotype) exhibit circadian rhythmicity in the activity and mRNA abundance for 1-aminocyclopropane-1-carboxylic acid oxidase (EC 1.4.3). The steady-state mRNA levels and enzymatic activity levels fluctuated with a period of approximately 24 h and reached their maxima by the middle of the light phase and minima by the middle of the dark phase. The oscillations showed damping under constant light, constant dark and constant temperature conditions, indicating that the rhythm is entrained by an external signal. The results indicate that light/dark cycles have greater entraining effects than temperature cycles. A 15-min red light pulse, but not a blue light pulse, could reset rhythm in continuous darkness, suggesting the possible role of a red-light signal transduction pathway in the circadian regulation of 1-aminocyclopropane-1-carboxylic acid oxidase.
长柱繁缕（草原生态型）的茎和叶组织在1-氨基环丙烷-1-羧酸氧化酶（EC 1.4.3）的活性和mRNA丰度方面表现出昼夜节律性。稳态mRNA水平和酶活性水平以约24小时的周期波动，在光照阶段中期达到最大值，在黑暗阶段中期达到最小值。在恒定光照、恒定黑暗和恒定温度条件下，振荡表现出阻尼，表明该节律受外部信号的调节。结果表明，光/暗循环比温度循环具有更大的调节作用。一个15分钟的红光脉冲，但不是蓝光脉冲，可以在持续黑暗中重置节律，这表明红光信号转导途径可能在1-氨基环丙烷-1-羧酸氧化酶的昼夜调节中发挥作用。
