Kathiresan A, Nagarathna K C, Moloney M M, Reid D M, Chinnappa C C
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Plant Mol Biol. 1998 Jan;36(2):265-74. doi: 10.1023/a:1005994118535.
Using degenerate oligonucleotides that correspond to conserved amino acid residues of known 1-aminocyclopropane-1-carboxylic acid (ACC) synthases, we cloned a genomic fragment that encodes ACC synthase in Stellaria longipes. Southern analysis suggests that ACC synthase is encoded by a small gene family comprising about 4 members. We isolated four unique ACC synthase cDNA clones under different growth conditions from alpine and prairie ecotypes of S. longipes. Northern analyses suggest that ACC synthase genes are differentially and synergistically regulated by photoperiod and temperature. Such differential regulation of ACC synthase genes positively correlate with the levels of ACC and ethylene. Since ethylene has previously been shown to partly control the stem elongation plasticity in S. longipes, we propose that differential regulation of ACC synthase genes may represent one of the underlying molecular mechanisms of phenotypic plasticity in S. longipes.
利用与已知的1-氨基环丙烷-1-羧酸（ACC）合酶保守氨基酸残基相对应的简并寡核苷酸，我们克隆了一个在长柱繁缕中编码ACC合酶的基因组片段。Southern分析表明，ACC合酶由一个约含4个成员的小基因家族编码。我们在不同生长条件下从长柱繁缕的高山和草原生态型中分离出四个独特的ACC合酶cDNA克隆。Northern分析表明，ACC合酶基因受光周期和温度的差异协同调控。ACC合酶基因的这种差异调控与ACC和乙烯的水平呈正相关。由于先前已表明乙烯部分控制长柱繁缕茎伸长的可塑性，我们提出ACC合酶基因的差异调控可能代表长柱繁缕表型可塑性的潜在分子机制之一。
