Nakagawa O, Fujisawa K, Ishizaki T, Saito Y, Nakao K, Narumiya S
Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.
FEBS Lett. 1996 Aug 26;392(2):189-93. doi: 10.1016/0014-5793(96)00811-3.
We recently identified a novel human protein kinase, p160 ROCK, as a putative downstream target of the small GTPase Rho. Using the human ROCK cDNA as a probe, we isolated cDNA of two distinct, highly related sequences from mouse libraries. One encoded a mouse counterpart of human ROCK (ROCK-I), and the other encoded a novel ROCK-related kinase (ROCK-II). Like ROCK/ROCK-I, ROCK-II also bound to GTP-Rho selectively. ROCK-I mRNA was ubiquitously expressed except in the brain and muscle, whereas ROCK-II mRNA was expressed abundantly in the brain, muscle, heart, lung and placenta. These results suggest that at least two ROCK isoforms are present in a single species and play distinct roles in Rho-mediated signalling pathways.
我们最近鉴定出一种新型人类蛋白激酶p160 ROCK，它可能是小GTP酶Rho的下游靶点。我们使用人类ROCK cDNA作为探针，从小鼠文库中分离出两个不同但高度相关序列的cDNA。一个编码人类ROCK的小鼠对应物（ROCK-I），另一个编码一种新型ROCK相关激酶（ROCK-II）。与ROCK/ROCK-I一样，ROCK-II也选择性地与GTP-Rho结合。ROCK-I mRNA除在脑和肌肉中外普遍表达，而ROCK-II mRNA在脑、肌肉、心脏、肺和胎盘中大量表达。这些结果表明，在单一物种中至少存在两种ROCK亚型，它们在Rho介导的信号通路中发挥不同作用。
