Takahashi N, Tuiki H, Saya H, Kaibuchi K
Central Laboratories for Key Technology, Kirin Brewery Company Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236, Japan.
Genomics. 1999 Jan 15;55(2):235-7. doi: 10.1006/geno.1998.5344.
Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. To elucidate the role of ROCK II in human disease, we have cloned a 4363-bp cDNA encoding human ROCK II. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Radiation hybrid mapping has shown that the human ROCK II gene is located very close to the highly polymorphic marker D2S359.
丝氨酸/苏氨酸激酶ROCK II/ Rho激酶是ROCK I的一种同工酶，是小GTP酶Rho的作用靶点之一。ROCK II调节肌动蛋白应激纤维和粘着斑的形成、胞质分裂、平滑肌收缩以及c-fos血清反应元件的激活。为了阐明ROCK II在人类疾病中的作用，我们克隆了一个编码人ROCK II的4363 bp cDNA。序列分析表明，人ROCK II含有1388个氨基酸残基，计算分子量约为161 kDa。荧光原位杂交分析表明，人ROCK II基因位于2号染色体p24区域。辐射杂种图谱分析表明，人ROCK II基因非常靠近高度多态性标记D2S359。
