Reinshagen M, Patel A, Sottili M, French S, Sternini C, Eysselein V E
Inflammatory Bowel Disease Center, Harbor-University of California, Los Angeles Medical Center, Torrance 90509, USA.
Am J Physiol. 1996 Jan;270(1 Pt 1):G79-86. doi: 10.1152/ajpgi.1996.270.1.G79.
The influence of sensory nerves on inflammation and healing was studied in a rat colitis model at different stages of inflammation. Studies were performed in animals with and without ablation of sensory nerves, which was achieved by pretreatment with the neurotoxin capsaicin. Colitis was induced by a rectal enema containing trinitrobenzenesulfonic acid (50 mg/kg) in 50% ethanol. Severity of inflammation was markedly increased 3 and 7 days after induction of colitis in the capsaicin-pretreated group compared with the vehicle group as determined by a macroscopic damage score (at 3 days, 12.0 +/- 0.7 vs. 7.5 +/- 1.5; at 7 days, 12.2 +/- 0.8 vs. 6.5 +/- 0.8; P < 0.05), by histology (ulceration score at 3 days, 82 +/- 12 vs. 40 +/- 11%; at 7 days, 92 +/- 5 vs. 46 +/- 13%; P < 0.05), and by myeloperoxidase activity (at 3 days, 133 +/- 30 vs. 42 +/- 14 U/mg protein; at 7 days, 76 +/- 11 vs. 39 +/- 11 U/mg protein; P < 0.05). There was no significant difference in the severity of colitis 14 and 21 days after induction of colitis between the capsaicin-pretreated group and the vehicle group. These data suggest that, in this model, sensory nerves have an important protective function in the acute and subacute phases of inflammation but do not seem to play a significant role in the later stages of chronic inflammation.
在大鼠结肠炎模型的不同炎症阶段，研究了感觉神经对炎症和愈合的影响。对有和没有感觉神经消融的动物进行了研究，感觉神经消融通过用神经毒素辣椒素预处理来实现。结肠炎通过在50%乙醇中直肠灌注含三硝基苯磺酸（50mg/kg）诱导。与载体组相比，辣椒素预处理组在结肠炎诱导后3天和7天，炎症严重程度明显增加，这通过宏观损伤评分确定（3天时，12.0±0.7对7.5±1.5；7天时，12.2±0.8对6.5±0.8；P<0.05），通过组织学（3天时溃疡评分，82±12对40±11%；7天时，92±5对46±13%；P<0.05），以及通过髓过氧化物酶活性（3天时，133±30对42±14U/mg蛋白；7天时，76±11对39±11U/mg蛋白；P<0.05）。辣椒素预处理组和载体组在结肠炎诱导后14天和21天，结肠炎严重程度没有显著差异。这些数据表明，在该模型中，感觉神经在炎症的急性和亚急性期具有重要的保护功能，但在慢性炎症后期似乎不发挥显著作用。
