Reinshagen M, Patel A, Sottili M, Nast C, Davis W, Mueller K, Eysselein V
Inflammatory Bowel Disease Center, Harbor-UCLA Medical Center, Torrance.
Gastroenterology. 1994 May;106(5):1208-14. doi: 10.1016/0016-5085(94)90011-6.
BACKGROUND/AIMS: Sensory nerves appear to have a protective effect against acute injury in the gastric mucosa. Their function in the intestine is unclear.
In this study an immune-complex model of colitis was used to induce inflammation in the distal colon with and without functional ablation of sensory neurons by capsaicin pretreatment.
Colitis was more severe in the capsaicin-pretreated group than in the vehicle group 48 and 96 hours after induction of colitis. Neutrophil infiltration, expressed as inflammatory index, was significantly increased to 4.25 +/- 0.4 vs. 1.83 +/- 0.5 at 48 hours and to 2.66 +/- 0.6 vs. 1.65 +/- 0.3 at 96 hours in the capsaicin group and the vehicle group, respectively. The microscopic ulcer index also was significantly increased in the capsaicin-pretreated group compared with the vehicle group (63.3 +/- 10.6 vs. 3.3 +/- 2.4 at 48 hours, 20.0 +/- 8.4 vs. 1.5 +/- 1.1 at 96 hours). Immunoreactive substance P (SP) and calcitonin gene-related peptide (CGRP) contents were decreased in extracts of inflamed compared with uninflamed colon.
These data suggest that sensory neurons have a protective role in an acute rabbit model of experimental colitis by release of sensory neuropeptides (SP, CGRP), which may modulate vascular tone and mucosal blood flow.
背景/目的：感觉神经似乎对胃黏膜急性损伤具有保护作用。其在肠道中的功能尚不清楚。
在本研究中，采用免疫复合物诱导的结肠炎模型，在有或没有通过辣椒素预处理使感觉神经元功能缺失的情况下，诱发远端结肠炎症。
诱导结肠炎后48小时和96小时，辣椒素预处理组的结肠炎比赋形剂组更严重。以炎症指数表示的中性粒细胞浸润在辣椒素组和赋形剂组中分别在48小时时显著增加至4.25±0.4对1.83±0.5，在96小时时增加至2.66±0.6对1.65±0.3。与赋形剂组相比，辣椒素预处理组的显微镜下溃疡指数也显著增加（48小时时为63.3±10.6对3.3±2.4，96小时时为20.0±8.4对1.5±1.1）。与未发炎的结肠相比，发炎结肠提取物中的免疫反应性P物质（SP）和降钙素基因相关肽（CGRP）含量降低。
这些数据表明，在实验性结肠炎的急性兔模型中，感觉神经元通过释放感觉神经肽（SP、CGRP）发挥保护作用，这些神经肽可能调节血管张力和黏膜血流。
