Tseng J F, Ryan I P, Milam T D, Murai J T, Schriock E D, Landers D V, Taylor R N
Division of Reproductive Endocrinology, University of California, San Francisco 94143, USA.
J Clin Endocrinol Metab. 1996 Mar;81(3):1118-22. doi: 10.1210/jcem.81.3.8772585.
An in vitro model developed to compare human endometrial and endometriosis stromal cells was used to examine basal and stimulated expression of interleukin (IL-6). Stromal cells isolated from normal endometrium (NE) exhibited the lowest level of IL-6 secretion (84 pg/10(6) cells-48 h), whereas those cells isolated from endometriosis implants (EI) secreted the highest concentration of this inflammatory cytokine (46,284 pg/10(5) cells-48 h; P < 0.01). Eutopic endometrial stromal cells from women with endometriosis (EE) expressed an intermediate concentration of IL-6 (831 pg/10(6) cells-48 h). Stimulation of the various cultures with IL-1 beta dramatically augmented stromal cell production of IL-6. The mean concentrations of stimulated IL-6 secretion were 16,257, 37,800, and 264,290 pg/10(5) cells-48 h for NE, EE, and EI cells, respectively (P < 0.03). Exposure of the cell cultures to 10 nmol/L estradiol had little direct effect on IL-6 production. The results indicate that endometrial stromal cells isolated from tissues of women with and without endometriosis express IL-6 under basal and cytokine-stimulated conditions. Differential responsiveness among the three cell sources indicates that NE, EE, and EI cells have intrinsic quantitative differences in cytokine regulation.
为比较人子宫内膜和子宫内膜异位症基质细胞而建立的体外模型用于检测白细胞介素（IL-6）的基础表达和刺激表达。从正常子宫内膜（NE）分离的基质细胞IL-6分泌水平最低（84 pg/10⁶细胞 - 48小时），而从子宫内膜异位症植入物（EI）分离的细胞分泌这种炎性细胞因子的浓度最高（46,284 pg/10⁵细胞 - 48小时；P < 0.01）。患有子宫内膜异位症的女性的在位子宫内膜基质细胞（EE）表达的IL-6浓度处于中等水平（831 pg/10⁶细胞 - 48小时）。用IL-1β刺激各种培养物可显著增加基质细胞IL-6的产生。对于NE、EE和EI细胞，刺激后IL-6分泌的平均浓度分别为16,257、37,800和264,290 pg/10⁵细胞 - 48小时（P < 0.03）。将细胞培养物暴露于10 nmol/L雌二醇对IL-6的产生几乎没有直接影响。结果表明，从患有和未患有子宫内膜异位症的女性组织中分离的子宫内膜基质细胞在基础条件和细胞因子刺激条件下均表达IL-6。三种细胞来源之间的差异反应性表明，NE、EE和EI细胞在细胞因子调节方面存在内在的定量差异。
