Gravenor I, Horton T L, Ritchie P, Flint E, Horton J D
Department of Biological Sciences, University of Durham, UK.
Dev Comp Immunol. 1995 Nov-Dec;19(6):507-23. doi: 10.1016/0145-305x(95)00030-w.
Recently generated anti-Xenopus T cell monoclonal antibodies (mAbs) to the 120 kDa XTLA-1 determinant and against the putative CD5 and CD8 homologues, together with anti-IgM and anti-MHC class II mAbs, are used in dual colour flow cytometric experiments to characterize cell surface antigenic expression on lymphocytes in thymus and spleen of Xenopus laevis during larval and early adult life and also in metamorphosis-inhibited animals. Histological confirmation of T cell emergence early in larval ontogeny is supplied by cryostat sections stained for CD8. Five-day thymectomy, i.e. prior to T-lineage cell differentiation in the thymus, abolishes T cell marker expression in the spleen for up to 1 year. Moreover, late larval (20 days) or early adult (3 months) thymectomy (i.e. removal after peripheralization of T cells has occurred) also leads to severe depletion of mAb-defined T cells in the spleen.
最近产生的针对120 kDa XTLA-1决定簇以及假定的CD5和CD8同源物的抗非洲爪蟾T细胞单克隆抗体（mAb），连同抗IgM和抗MHC II类mAb，被用于双色流式细胞术实验，以表征非洲爪蟾幼体和成年早期以及变态抑制动物胸腺和脾脏中淋巴细胞的细胞表面抗原表达。通过对CD8进行染色的低温恒温器切片提供了幼体个体发育早期T细胞出现的组织学确认。五天胸腺切除术，即在胸腺中T系细胞分化之前进行，可使脾脏中的T细胞标志物表达在长达1年的时间内消失。此外，幼体晚期（20天）或成年早期（3个月）胸腺切除术（即在T细胞外周化后进行切除）也会导致脾脏中mAb定义的T细胞严重耗竭。
