Yuan Y V, Kitts D D, Godin D V
Department of Food Science, Faculty of Agricultural Sciences, University of British Columbia, Vancouver, Canada.
Can J Physiol Pharmacol. 1996 Mar;74(3):290-7.
Heart and red blood cell endogenous antioxidant status and plasma lipids were investigated in hypertensive, 14-week-old spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats fed a standard commercial rat chow. Specific heart and red blood cell antioxidant enzyme activities, as well as the susceptibility of tissues to H2O2-induced glutathione (GSH) depletion and lipid peroxidation, were measured. Systolic blood pressure in SHR was greater than in WKY rats at 13 weeks of age (197 +/- 12 vs. 132 +/- 14 mmHg (1 mmHg = 133.3 Pa); p < or = 0.05), confirming the presence of hypertension in SHR. Red blood cell catalase (CAT) and superoxide dismutase (SOD) activities were greater (p < or = 0.05) in SHR than WKY rats. Red blood cell CAT activity was positively correlated (r = +0.634; p = 0.026) with SOD, which in turn was correlated (r = +0.709; p = 0.049) with systolic blood pressure. Heart SOD activity was higher (p < or = 0.05) in SHR, while glutathione reductase (GSSG-Red) activity was lower (p < or = 0.05) than in WKY rats. This reduced ability to recycle GSH in the heart coincided with greater (p < or = 0.05) levels of H2O2-induced lipid oxidation products in SHR. Plasma total cholesterol and triacylglycerol levels were lower (p < or = 0.05) in SHR than WKY rats, with no visible signs of atherosclerosis in either SHR or WKY rats. In summary, hypertension in SHR was associated with alterations in antioxidant enzyme profiles of red blood cells and heart, with the latter showing an increased susceptibility to in vitro lipid oxidation. Although hypertension is a recognized factor in the development of human atherosclerosis, spontaneously hypertensive rats did not exhibit signs of aortic plaque, reflecting the resistance of this species to the development of atherosclerosis.
在喂食标准市售大鼠饲料的14周龄高血压自发性高血压大鼠（SHR）和血压正常的Wistar-Kyoto（WKY）大鼠中，研究了心脏和红细胞内源性抗氧化状态及血脂情况。测定了心脏和红细胞特异性抗氧化酶活性，以及组织对过氧化氢（H2O2）诱导的谷胱甘肽（GSH）消耗和脂质过氧化的敏感性。13周龄时，SHR的收缩压高于WKY大鼠（197±12 vs. 132±14 mmHg（1 mmHg = 133.3 Pa）；p≤0.05），证实SHR存在高血压。SHR的红细胞过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性高于WKY大鼠（p≤0.05）。红细胞CAT活性与SOD呈正相关（r = +0.634；p = 0.026），而SOD又与收缩压相关（r = +0.709；p = 0.049）。SHR的心脏SOD活性较高（p≤0.05），而谷胱甘肽还原酶（GSSG-Red）活性低于WKY大鼠（p≤0.05）。心脏中这种回收GSH能力的降低与SHR中H2O2诱导的脂质氧化产物水平升高（p≤0.05）相吻合。SHR的血浆总胆固醇和三酰甘油水平低于WKY大鼠（p≤0.05），SHR和WKY大鼠均无明显动脉粥样硬化迹象。总之，SHR中的高血压与红细胞和心脏抗氧化酶谱的改变有关，后者对体外脂质氧化的敏感性增加。虽然高血压是人类动脉粥样硬化发展的一个公认因素，但自发性高血压大鼠未表现出主动脉斑块迹象，这反映了该物种对动脉粥样硬化发展的抗性。
