Jia Han, Liu Ji Wen, Ufur Halmurat, He Geng Sheng, Liqian Hai, Chen Peipei
Department of Nutrition and Food Hygiene, College of Public Health, Xinjiang Medical University, Urumqi, China.
Pharmacogn Mag. 2011 Jan;7(25):19-24. doi: 10.4103/0973-1296.75885.
To evaluate the antihypertensive effect of Xinjiang red raspberry fruit ethyl acetate extract (EER) on spontaneously hypertensive rats (SHR) and its possible mechanism from antioxidant perspective.
The SHR rats were randomly divided into 3 groups, and treated with EER low dose (EERL, 100 mg/kg/d), high dose (EERH, 200 mg/kg/d), and water (SHR) through gastric gavage daily for 5 weeks. Another 8 age-matched male Wistar-Kyoto rats were used as normotensive group (WKY). The systolic blood pressure (SBP) was measured by noninvasive tail-cuff method once a week. At the end of the treatment, blood samples were collected and serum concentrations of nitric oxide (NO), superoxide dismutase (SOD), malondialchehyche (MDA), and plasma endothelin (ET) were determined.
Treatment of SHR rats with EER lowered the blood pressure compared with that treated with water (SHR), and the high dose showed more significant reduction in blood pressure. Treatment of SHR rats with EER increased serum NO and SOD levels and lowered ET and MDA levels. As compared with control group, NO levels were increased significantly in EERL (P < 0.01), SOD was elevated more significantly in both EERL and EERH (P < 0.01); MDA was decreased significantly in EERH group (P < 0.05), whereas plasma ET decreased more significantly in the EERH group (P < 0.05).
The red raspberry extracts demonstrated a dose-dependent antihypertensive effects in SHR and this may be related to increased NO activation and improved vascular endothelial dysfunction via antioxidation. These results confirmed that raspberries rich in polyphenols have potential cardiovascular protective effects.
从抗氧化角度评价新疆红树莓果实乙酸乙酯提取物（EER）对自发性高血压大鼠（SHR）的降压作用及其可能机制。
将SHR大鼠随机分为3组，分别每日经胃灌胃给予EER低剂量组（EERL，100mg/kg/d）、高剂量组（EERH，200mg/kg/d）和水（SHR组），持续5周。另取8只年龄匹配的雄性Wistar-Kyoto大鼠作为正常血压组（WKY）。每周采用无创尾套法测量收缩压（SBP）。治疗结束时，采集血样并测定血清一氧化氮（NO）、超氧化物歧化酶（SOD）、丙二醛（MDA）浓度以及血浆内皮素（ET）水平。
与给予水的SHR大鼠（SHR组）相比，用EER治疗SHR大鼠可降低血压，且高剂量组降压效果更显著。用EER治疗SHR大鼠可提高血清NO和SOD水平，降低ET和MDA水平。与对照组相比，EERL组NO水平显著升高（P<0.01），EERL组和EERH组SOD升高更显著（P<0.01）；EERH组MDA显著降低（P<0.05），而EERH组血浆ET降低更显著（P<0.05）。
红树莓提取物对SHR具有剂量依赖性降压作用，这可能与通过抗氧化增加NO活性和改善血管内皮功能障碍有关。这些结果证实富含多酚的树莓具有潜在的心血管保护作用。
