Bannon A W, Curzon P, Gunther K L, Decker M W
Dept. 47W, Abbott Laboratories, Abbott Park, IL 60064-3500, USA.
Brain Res. 1996 Apr 29;718(1-2):25-36. doi: 10.1016/0006-8993(95)01568-x.
In this study, the effects intraseptal injections of the selective cholinergic immunotoxin, 192-IgG-saporin, were investigated in mature (6-month-old) and aged (24-26-month-old) male Long-Evans rats. Ten days following intraseptal injection of either 192-IgG-saporin or saline, testing began in a battery of behavioral tests modulated by the septohippocampal system including two versions of the Morris water maze (i.e. submerged platform task, and 2-platform spatial discrimination), inhibitory avoidance, and pre-pulse inhibition of acoustic startle. In both mature and aged rats, intraseptal injection of 192-IgG-saporin selectively reduced ChAT activity in the hippocampus and posterior cingulate cortex, without affecting ChAT activity of amygdala or parietal cortex. In general, in all of the behavioral tests analyzed, intraseptal 192-IgG-saporin treatment had no effect in mature animals. Age-related deficits were observed in the spatial memory tasks, however this impairment was largely a function of the poor performance of aged rats treated with the toxin. In addition, an increase in the response to an acoustic startle was found in aged rats treated with 192-IgG-saporin. Thus, although intraseptal injection of 192-IgG-saporin produced similar reductions of ChAT activity, performance of mature and aged rats in tasks believed to be modulated by the septohippocampal pathway tended to be differentially affected in mature and aged rats.
在本研究中，我们对成熟（6 月龄）和老龄（24 - 26 月龄）雄性 Long-Evans 大鼠进行了研究，观察了隔区内注射选择性胆碱能免疫毒素 192-IgG-皂草素的效果。在隔区内注射 192-IgG-皂草素或生理盐水 10 天后，开始进行一系列由隔海马系统调节的行为测试，包括两种版本的 Morris 水迷宫（即水下平台任务和双平台空间辨别）、抑制性回避以及听觉惊吓的前脉冲抑制。在成熟和老龄大鼠中，隔区内注射 192-IgG-皂草素均选择性降低了海马和后扣带回皮质中的 ChAT 活性，而不影响杏仁核或顶叶皮质的 ChAT 活性。总体而言，在所有分析的行为测试中，隔区内注射 192-IgG-皂草素对成熟动物没有影响。然而，在空间记忆任务中观察到了与年龄相关的缺陷，但这种损伤很大程度上是毒素处理的老龄大鼠表现不佳的结果。此外，在用 192-IgG-皂草素处理的老龄大鼠中发现对听觉惊吓的反应增加。因此，尽管隔区内注射 192-IgG-皂草素产生了类似的 ChAT 活性降低，但成熟和老龄大鼠在被认为由隔海马通路调节的任务中的表现，在成熟和老龄大鼠中往往受到不同的影响。
