Janis L S, Glasier M M, Fulop Z, Stein D G
Institute of Animal Behavior, Rutgers University, Newark, NJ 07102, USA.
Behav Brain Res. 1998 Jan;90(1):23-34. doi: 10.1016/s0166-4328(97)00078-8.
The involvement of the cholinergic septohippocampal system in strategies used to reach a spatial goal was examined by functionally inactivating this system with infusions of 192 IgG saporin, a potent cholinergic immunotoxin. Rats were initially trained on a win-shift radial arm maze (RAM) task and then given injections of either 192 IgG saporin (LES) or saline vehicle (CON) into the medial septum and vertical limb of the diagonal band. Rats were then retested postoperatively on the RAM to assess whether allocentric spatial strategies used to solve the task were impaired. The results indicated that injections of 192 IgG saporin into the septum of rats produced deficits in allocentric strategies used to locate the spatial goal when retested. In addition, place and response learning was also examined in a modified version of the Morris water maze task. In this task, rats with cholinergic lesions were mildly impaired in their ability to learn a place response. In order to clarify further whether rats may have been relying on allocentric or egocentric learning strategies to locate the platform, a probe trial was given on the final test day in which the visible platform was moved to a new location. Control rats swam either to the new platform location or the old platform location indicating the use of both an allocentric and egocentric response. However, rats with the cholinergic septal lesions swam to the new platform location indicating an egocentric response. Taken together, these results suggest that selective cholinergic lesions of the septum produce deficits in spatial strategies used to locate a spatial goal.
通过向胆碱能隔海马系统注射192 IgG皂草素（一种有效的胆碱能免疫毒素）使其功能失活，来研究该系统在实现空间目标所采用策略中的作用。大鼠最初接受放射状臂迷宫（RAM）任务训练，然后向其内侧隔区和斜角带垂直支注射192 IgG皂草素（LES组）或生理盐水（CON组）。随后对大鼠进行术后RAM复测，以评估用于解决任务的以自我为中心的空间策略是否受损。结果表明，对大鼠隔区注射192 IgG皂草素后，复测时用于定位空间目标的以自我为中心的策略出现缺陷。此外，还在改良版的莫里斯水迷宫任务中检测了位置学习和反应学习。在该任务中，胆碱能损伤的大鼠在学习位置反应的能力上有轻微受损。为了进一步阐明大鼠是否可能一直依赖以自我为中心或自我中心的学习策略来定位平台，在最后测试日进行了一次探测试验，将可见平台移至新位置。对照大鼠游向新平台位置或旧平台位置，表明它们同时使用了以自我为中心和自我中心的反应。然而，胆碱能隔区损伤的大鼠游向新平台位置，表明是自我中心反应。综上所述，这些结果表明隔区的选择性胆碱能损伤会导致用于定位空间目标的空间策略出现缺陷。
