Atkins T W, Tighe B J
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK.
J Biomater Sci Polym Ed. 1996;7(9):759-68. doi: 10.1163/156856296x00101.
A prescreen of the in vitro cytotoxicity of both the primary fabrication components and potential leachables from a bead-formed macroporous poly(2-hydroxyethyl methacrylate), (pHEMA) matrix has been carried out using INVITTOX Neutral red and Kenacid blue R dye binding methods. Of the eluants obtained from 24, 48, and 72-h incubated beads, only the 72-h eluant produced a greater than 20% (ID20) inhibition of 3T3-L1 cell proliferation with values of 20.98 +/- 2.33% and 21.41 +/- 1.37% inhibition for the Neutral red and Kenacid blue R binding methods, respectively. ID50 values for the fabrication components obtained using the Kenacid blue R method were generally higher than those obtained by the Neutral red assay, although the ranking of the chemicals in terms of their relative cytotoxicities was identical by both methods, i.e. ethylene glycol dimethacrylate > uranyl nitrate > purified HEMA > n-hexane > ethylene glycol (mmol 1(-1)). Whilst extended washing of finished PHEMA beads in water will reduce their acute in vitro cytotoxicity, this will only be achieved with some loss of previously encapsulated water soluble macromolecules.
使用INVITTOX中性红和肯纳酸蓝R染料结合法对珠状大孔聚甲基丙烯酸2-羟乙酯（pHEMA）基质的主要制备成分及其潜在可浸出物的体外细胞毒性进行了预筛选。从经过24、48和72小时孵育的珠子中获得的洗脱液中，只有72小时的洗脱液对3T3-L1细胞增殖产生了大于20%（ID20）的抑制作用，中性红和肯纳酸蓝R结合法的抑制率分别为20.98±2.33%和21.41±1.37%。使用肯纳酸蓝R法获得的制备成分的ID50值通常高于通过中性红测定法获得的值，尽管两种方法对化学物质相对细胞毒性的排名相同，即二甲基丙烯酸乙二醇酯>硝酸铀酰>纯化的甲基丙烯酸羟乙酯>正己烷>乙二醇（mmol l-1）。虽然在水中对成品PHEMA珠子进行长时间洗涤会降低其急性体外细胞毒性，但这只能在一定程度上实现，同时会损失一些先前包封的水溶性大分子。
