Zhang Li, Zheng Gen-Jian, Guo Ya-Tong, Zhou Lan, Du Jie, He Hong
Department of Orthodontics, Hubei-MOST KLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Department of Stomatology, The Affiliated Hospital of Hainan Medical College, Hainan Medical University, Haikou 570102, China.
Asian Pac J Trop Med. 2014 Feb;7(2):136-40. doi: 10.1016/S1995-7645(14)60009-2.
To prepare a novel biodegradable poly(2-hydroxyethylmethacrilate) (pHEMA) hydrogel as tissue engineering scaffold.
The pHEMA hydrogel was synthesized by microwave-assisted polymerization using 2-hydroxyethyl methacrylate (HEMA) as the raw material, potassium persulfate as the initiator, and PCLX as the cross-linking additive. The hydrogels was characterized with FTIR and NMR spectroscopy. The physical and chemical properties of the prepared hydrogel were evaluated, and its degradation performance was tested. The cytotoxicity of the optimum composite hydrogel was measured by an MTT assay to confirm the feasibility of its use in tissue engineering.
The optimum conditions under which the hydrogel was prepared by microwave-assisted polymerization are as follows: 1.5 g cross-linking additive, 0.3 g initiator, reaction temperature of 80 °C, and microwave power of 800 W. Degradation studies showed good degradation profiles with 75% in 17 days. Additionally, the hydrogels did not elicit any cytotoxic response in in vitro cytotoxic assays.
A biodegradable pHEMA hydrogel was successfully prepared by microwave-assisted polymerization, as confirmed from FTIR and NMR results. The hydrogel shows promising applications in tissue engineering, and its healing ability and biocompatibility will be evaluated in detail in the future.
制备一种新型可生物降解的聚甲基丙烯酸2-羟乙酯（pHEMA）水凝胶作为组织工程支架。
以甲基丙烯酸2-羟乙酯（HEMA）为原料，过硫酸钾为引发剂，PCLX为交联添加剂，通过微波辅助聚合法合成pHEMA水凝胶。采用傅里叶变换红外光谱（FTIR）和核磁共振光谱（NMR）对水凝胶进行表征。评估所制备水凝胶的物理和化学性质，并测试其降解性能。通过MTT法测定最佳复合水凝胶的细胞毒性，以确认其在组织工程中应用的可行性。
微波辅助聚合法制备水凝胶的最佳条件如下：交联添加剂1.5 g、引发剂0.3 g、反应温度80℃、微波功率800 W。降解研究表明降解情况良好，17天内降解率达75%。此外，在体外细胞毒性试验中，水凝胶未引发任何细胞毒性反应。
通过FTIR和NMR结果证实，采用微波辅助聚合法成功制备了一种可生物降解的pHEMA水凝胶。该水凝胶在组织工程中显示出有前景的应用，其愈合能力和生物相容性将在未来进行详细评估。
