Onning G, Wang Q, Weström B R, Asp N G, Karlsson B W
Department of Applied Nutrition and Food Chemistry, Chemical Center, Lund University, Sweden.
Br J Nutr. 1996 Jul;76(1):141-51. doi: 10.1079/bjn19960016.
The aim of the present study was to investigate whether oat saponins (avenacosides A and B) have any effect on the permeability of the rat intestine to actively and passively transported markers in vitro and in vivo. Intestinal segments were mounted in modified Ussing chambers, and the passage of the different marker compounds from the mucosal to the serosal side was measured for 120 min. Avenacosides (1 mg/ml) gave a significantly higher passage of the macromolecule ovalbumin and there was a tendency to increased passage of [14C]D-mannitol and [51Cr]EDTA. On the other hand, the saponins did not affect the active transport of [3H]methyl glucose. When rats were given saponins (40 mg/kg body weight) together with markers by gastric intubation, the passage of [51Cr]EDTA into blood and urine was somewhat reduced. For the macromolecule bovine serum albumin, no evident effect on the passage was observed in the presence of saponins. Thus, in contrast to the in vitro results, the in vivo marker passage seemed to be unaffected or even reduced in the presence of avenacosides. The study shows that saponins can affect the permeability of the rat intestine. However, this effect needs further investigation in vivo, especially regarding proteins.
本研究的目的是调查燕麦皂苷（燕麦皂苷A和B）在体外和体内对大鼠肠道主动和被动转运标志物通透性的影响。将肠段安装在改良的尤斯灌流小室中，测量不同标志物化合物从黏膜侧到浆膜侧120分钟的转运情况。燕麦皂苷（1毫克/毫升）使大分子卵清蛋白的转运显著增加，并且[14C]D-甘露糖醇和[51Cr]乙二胺四乙酸的转运有增加的趋势。另一方面，皂苷对[3H]甲基葡萄糖的主动转运没有影响。当通过胃插管给大鼠注射皂苷（40毫克/千克体重）并同时给予标志物时，[51Cr]乙二胺四乙酸进入血液和尿液的转运有所减少。对于大分子牛血清白蛋白，在存在皂苷的情况下未观察到对转运的明显影响。因此，与体外结果相反，在存在燕麦皂苷的情况下，体内标志物转运似乎未受影响甚至有所降低。该研究表明皂苷可影响大鼠肠道的通透性。然而，这种影响在体内还需要进一步研究，尤其是关于蛋白质方面。
