Holm-Hansen C, Ayehunie S, Johansson B, Nkya W, Shao J, Haukenes G
Centre for International Health, Gade Institute, University of Bergen, Norway.
APMIS. 1996 Jun;104(6):459-64.
The polymerase chain reaction (PCR) and direct DNA sequencing were used to detect and characterize selected regions of the HIV-1 proviral genome in whole blood samples from Tanzania. Specific PCR amplification products were obtained in gag and/or env (gp41) regions from 15 of the 19 HIV-1 seropositive samples investigated. Env regions from 12 different amplificates were further characterized using the dideoxy sequencing method. Preliminary results indicate that, despite scattered nucleotide mismatches, HIV-1 gp41 amino acid sequences from Tanzania conform to the 1990 Los Alamos African consensus sequence and resemble the HIV-1 subtype A or D consensus sequences in the characterized regions.
采用聚合酶链反应（PCR）和直接DNA测序技术，对来自坦桑尼亚的全血样本中HIV-1前病毒基因组的选定区域进行检测和特征分析。在所研究的19份HIV-1血清阳性样本中，有15份在gag和/或env（gp41）区域获得了特异性PCR扩增产物。使用双脱氧测序法对12个不同扩增产物的env区域进行了进一步特征分析。初步结果表明，尽管存在零散的核苷酸错配，但来自坦桑尼亚的HIV-1 gp41氨基酸序列符合1990年洛斯阿拉莫斯非洲共识序列，并且在特征区域与HIV-1 A亚型或D亚型共识序列相似。
