Sutherland G R
Science. 1977 Jul 15;197(4300):265-6. doi: 10.1126/science.877551.
The observation of heritable fragile sites on human chromosomes prepared for lymphocyte cultures has been shown to depend on the type of tissue culture medium in which the lymphocytes are grown. The sites are observed at a much greater frequency when medium 199 is used than when RPMI 1640, Ham's F10, Eagle's (basal), and CMRL 1969 are used. One site on the X chromosome is of clinical significance in that it is a marker for X-linked mental retardation.
在为淋巴细胞培养准备的人类染色体上观察到的可遗传脆性位点，已表明取决于淋巴细胞生长所在的组织培养基类型。与使用RPMI 1640、哈姆氏F10、伊格尔氏（基础）培养基和CMRL 1969培养基时相比，使用199培养基时观察到这些位点的频率要高得多。X染色体上的一个位点具有临床意义，因为它是X连锁智力迟钝的一个标记。
