超氧化物歧化酶在人类卵巢和输卵管中的作用。
The role of superoxide dismutase in the human ovary and fallopian tube.
作者信息
Tamate K, Sengoku K, Ishikawa M
机构信息
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
出版信息
J Obstet Gynaecol (Tokyo 1995). 1995 Aug;21(4):401-9. doi: 10.1111/j.1447-0756.1995.tb01029.x.
OBJECTIVES
To investigate the localization of manganese superoxide dismutase (Mn-SOD) and copper-zinc superoxide dismutase (Cu, Zn-SOD) in the human ovary and fallopian tube, and to examine the role of superoxide radicals and SODs in the human ovulatory process.
METHODS
Using immunohistochemical methods, we studied the localization of SODs in 22 human ovaries, in 18 fallopian tubes, and in aspirated granulosa cells. We measured, by enzyme-linked immunosorbent assay, the concentrations of SODs in follicular fluid taken from 94 IVF patients.
RESULTS
Mn-SOD was found in granulosa, in theca and luteal cells and in fallopian tubes. Cu, Zn-SOD was localized in theca and luteal cells. The concentration of Cu, Zn-SOD in follicular fluid in the high-progesterone group (11.3 + 4.2 ng/ml) was significantly less than in the low-progesterone group (24.5 + 19.5) (p < 0.05).
CONCLUSION
Mn-SOD and Cu, Zn-SOD have different localizations and actions in human ovaries and fallopian tubes. The superoxide radical-SOD system might play an important role in ovulation and in the luteal function of the human ovary.
目的
研究锰超氧化物歧化酶（Mn-SOD）和铜锌超氧化物歧化酶（Cu, Zn-SOD）在人卵巢和输卵管中的定位，并探讨超氧阴离子自由基和超氧化物歧化酶在人类排卵过程中的作用。
方法
采用免疫组织化学方法，我们研究了22例人卵巢、18例输卵管及吸出的颗粒细胞中SOD的定位。通过酶联免疫吸附测定法，我们检测了94例体外受精患者卵泡液中SOD的浓度。
结果
在颗粒细胞、卵泡膜细胞、黄体细胞及输卵管中发现了Mn-SOD。Cu, Zn-SOD定位于卵泡膜细胞和黄体细胞。高孕酮组卵泡液中Cu, Zn-SOD的浓度（11.3 + 4.2 ng/ml）显著低于低孕酮组（24.5 + 19.5）（p < 0.05）。
结论
Mn-SOD和Cu, Zn-SOD在人卵巢和输卵管中具有不同的定位和作用。超氧阴离子自由基-SOD系统可能在人类排卵及卵巢黄体功能中起重要作用。
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