Green S, Dawson B T, Goodman C, Carey M F
School of Human Movement Studies, Queensland University of Technology, Brisbane, Australia.
Med Sci Sports Exerc. 1996 Mar;28(3):315-21. doi: 10.1097/00005768-199603000-00007.
Anaerobic ATP production in skeletal muscle and the accumulated oxygen deficit (O2D) incurred during an exhaustive cycle bout (duration = 173 +/- 24 s; intensity = 112 +/- 3% VO2peak), were determined in 10 male cyclists (mean +/- SD: VO2peak = 69.8 +/- 4.2 ml.kg-1.min-1). Anaerobic ATP production (mmol.kg-1 d.w.) was determined from changes in lactate, phosphocreatine, ATP, and ADP in vastus lateralis. Muscle buffer value and the activities of glycogen phosphorylase (PHOS), phosphofructokinase and citrate synthase (CS) were also determined. The anaerobic ATP production determined from measured muscle metabolites was 202.7 +/- 46.9 mmol.kg-1 d.w. and was correlated (P < or = 0.05) with muscle buffer value (r = 0.81), PHOS (r = 0.69) and the ratio of PHOS to CS activity (r = 0.77). The O2D was 55.2 +/- 10.3 ml O2 Eq.kg-1, but was not correlated (P > 0.05) with anaerobic ATP production (r = -0.38), buffer value (r = -0.50) or PHOS (r = -0.39). These latter findings could be explained by error in measuring the O2D and/or muscle anaerobic ATP production in well-trained cyclists.
对10名男性自行车运动员（平均值±标准差：最大摄氧量=69.8±4.2 ml·kg⁻¹·min⁻¹）进行了测定，以确定其骨骼肌中的无氧ATP生成以及在一次力竭性骑行运动（持续时间=173±24秒；强度=112±3%最大摄氧量）中产生的累积氧亏（O2D）。通过测定股外侧肌中乳酸、磷酸肌酸、ATP和ADP的变化来确定无氧ATP生成（mmol·kg⁻¹干重）。还测定了肌肉缓冲值以及糖原磷酸化酶（PHOS）、磷酸果糖激酶和柠檬酸合酶（CS）的活性。根据测量的肌肉代谢物确定的无氧ATP生成量为202.7±46.9 mmol·kg⁻¹干重，并且与肌肉缓冲值（r = 0.81）、PHOS（r = 0.69）以及PHOS与CS活性的比值（r = 0.77）相关（P≤0.05）。O2D为55.2±10.3 ml O2 Eq·kg⁻¹，但与无氧ATP生成（r = -0.38）、缓冲值（r = -0.50）或PHOS（r = -0.39）无相关性（P>0.05）。后面这些发现可能是由于在训练有素的自行车运动员中测量O2D和/或肌肉无氧ATP生成时存在误差所致。
