Vaalamo M, Weckroth M, Puolakkainen P, Kere J, Saarinen P, Lauharanta J, Saarialho-Kere U K
Department of Dermatology, Helsinki University Central Hospital, Finland.
Br J Dermatol. 1996 Jul;135(1):52-9.
The present study was carried out to characterize the patterns of expression of matrix metalloproteinases or their tissue inhibitor (TIMP-1) in normally healing, acute vs. chronic, skin wounds. In situ hybridization was performed to localize collagenase, stromelysin-1, stromelysin-2, matrilysin, urokinase plasminogen activator (uPA) and TIMP-1 mRNAs in 14 chronic venous ulcers and 10 normally healing wounds, representing different time points after wounding. Surgical wounds, made in piglets harvested at several time points, were studied as controls. Collagenase, stromelysin-1 and -2, as well as uPa, were expressed in keratinocytes in both acute and chronic wounds, while epithelial TIMP-1 mRNA was not detected in any chronic wound biopsies studied. However, TIMP-1 was expressed at the epithelial edges of both acute human and pig wounds. Our results suggest that the balance between metalloenzymes and their inhibitor TIMP-1, is disturbed, in poorly healing wounds.
本研究旨在表征基质金属蛋白酶或其组织抑制剂（TIMP-1）在正常愈合、急性与慢性皮肤伤口中的表达模式。对14例慢性静脉溃疡和10例正常愈合伤口进行原位杂交，以定位胶原酶、基质溶解素-1、基质溶解素-2、基质溶素、尿激酶型纤溶酶原激活剂（uPA）和TIMP-1 mRNA，这些伤口代表受伤后的不同时间点。在几个时间点采集的仔猪身上制造的手术伤口作为对照进行研究。胶原酶、基质溶解素-1和-2以及uPa在急性和慢性伤口的角质形成细胞中均有表达，而在所研究的任何慢性伤口活检中均未检测到上皮TIMP-1 mRNA。然而，TIMP-1在急性人类和猪伤口的上皮边缘均有表达。我们的结果表明，在愈合不良的伤口中，金属酶与其抑制剂TIMP-1之间的平衡受到干扰。
