Almeida R P, Vanet A, Witko-Sarsat V, Melchior M, McCabe D, Gabay J E
Division of International Medicine and Infectious Diseases, Cornell University Medical College, New York, New York 10021, USA.
Protein Expr Purif. 1996 Jun;7(4):355-66. doi: 10.1006/prep.1996.0053.
The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells.
人类中性粒细胞的嗜天青颗粒含有四种抗生素蛋白，即血清杀菌素，它们彼此之间以及与丝氨酸蛋白酶具有广泛的同源性。该家族的成员天青杀素是一种29 kDa的糖蛋白，具有广谱抗菌活性和对单核细胞的趋化活性。用携带天青杀素cDNA的杆状病毒载体转染昆虫细胞，产生了重组天青杀素蛋白。我们从感染细胞的培养基中纯化了重组天青杀素蛋白，并表明它保留了天然中性粒细胞衍生分子的抗菌活性。此外，我们提供的证据表明，重组天青杀素蛋白的一个49个氨基酸的区域是其从昆虫细胞分泌所必需的。
