Tong Y C, Hung Y C, Lin S N, Cheng J T
Department of Urology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China.
Urol Int. 1996;56(4):207-10. doi: 10.1159/000282843.
Clinically, a 59% prevalence of impotence was reported among diabetic male patients. Neurological, vascular, endocrinologic and psychological factors are probably involved. Previous reports with the rat model found deterioration of sexual behavior and reproductive function caused by streptozotocin (STZ)-induced diabetes. The purpose of the present study was to develop the dark-cycle video recording methodology for the observation of rat sexual activities and to study the effect of STZ-induced diabetes on sexual performances of the rat. Adult male Wistar rats were rendered diabetic with intraperitoneal injection of STZ (60 mg/kg body weight). In the 4th week a diabetic rat or a control rat was caged with an adult ovariectomized female rat during the dark cycle. The female had been brought into behavioral estrus with intramuscular injection of 0.1 mg estradiol benzoate 3 days before and 1.0 mg progesterone 3 h before testing. Infrared-light-illuminated video recording was performed to evaluate the sexual performances. The mounting latency and frequency, intromission latency and frequency, the hit rate as well as the post-ejaculatory period of the diabetic rats were significantly deteriorated when compared with the controls (p < 0.05). However, the ejaculatory latency showed no significant difference between the two groups (p > 0.05). In conclusion, it has been demonstrated that with this methodology, behavioral studies on nocturnal animals like the rat can be carried out conveniently. It was shown that the sexual arousal mechanism and copulation-ejaculatory mechanism were both depressed in STZ-induced diabetic rats. The same study model can be used for further pathophysiological and pharmacological researches on the sexual behaviors of diabetic rats.
临床上，据报道糖尿病男性患者中阳痿的患病率为59%。神经、血管、内分泌和心理因素可能都与之相关。先前关于大鼠模型的报告发现，链脲佐菌素（STZ）诱导的糖尿病会导致性行为和生殖功能恶化。本研究的目的是开发暗周期视频记录方法以观察大鼠的性活动，并研究STZ诱导的糖尿病对大鼠性行为表现的影响。成年雄性Wistar大鼠通过腹腔注射STZ（60mg/kg体重）诱导成为糖尿病大鼠。在第4周，将一只糖尿病大鼠或对照大鼠在暗周期与一只成年去卵巢雌性大鼠关在一个笼子里。在测试前3天，通过肌肉注射0.1mg苯甲酸雌二醇，测试前3小时肌肉注射1.0mg孕酮，使雌性大鼠进入行为发情期。进行红外光照明视频记录以评估性行为表现。与对照组相比，糖尿病大鼠的爬跨潜伏期和频率、插入潜伏期和频率、命中率以及射精后时期均显著恶化（p<0.05）。然而，两组之间的射精潜伏期没有显著差异（p>0.05）。总之，已证明使用这种方法可以方便地对大鼠等夜行性动物进行行为研究。结果表明，STZ诱导的糖尿病大鼠的性唤起机制和交配射精机制均受到抑制。相同的研究模型可用于对糖尿病大鼠性行为进行进一步的病理生理学和药理学研究。
