Abuín M, Clabby C, Martínez P, Goswami U, Flavin F, Wilkins N P, Houghton J A, Powell R, Sánchez L
Departamento de Biología Fundamental, Universidad de Santiago de Compostela, Lugo, Spain.
Genome. 1996 Aug;39(4):671-9. doi: 10.1139/g96-085.
A repetitive element was isolated from the genome of Atlantic salmon. Nucleotide sequence analysis revealed the existence of variant monomers that range in length from approximately 200 to 230 bp. Repeat monomers contain regions of cryptic simplicity, internal repetition, and long direct repeats with deletions and insertions between individual units. The repetitive element was shown to have a tandem unit arrangement and was estimated to occupy between two and three percent of the Atlantic salmon genome. Southern blot analysis revealed the repetitive element to be unique to Atlantic salmon and brown trout species. In situ hybridization analysis showed this element to be localized at the main nucleolar organizer region bearing chromosomes of Atlantic salmon (Salmo salar), AS cell line (derived from S. salar), and brown trout (Salmo trutta).
从大西洋鲑鱼的基因组中分离出一种重复元件。核苷酸序列分析揭示了存在长度约为200至230 bp的可变单体。重复单体包含隐蔽简单区域、内部重复以及各个单元之间存在缺失和插入的长直接重复序列。该重复元件显示具有串联单元排列，估计占大西洋鲑鱼基因组的2%至3%。Southern印迹分析表明该重复元件是大西洋鲑鱼和褐鳟物种所特有的。原位杂交分析显示该元件定位于大西洋鲑鱼（Salmo salar）、AS细胞系（源自S. salar）和褐鳟（Salmo trutta）的携带主要核仁组织区的染色体上。
